Expression of multiple alpha1-antitrypsin-like genes in hibernating species of the squirrel family

Gene
Nobuhiko TakamatsuT Shiba

Abstract

In the chipmunk, a mammalian hibernator, a 140 kDa protein complex found in the blood, drastically decreases in concentration during hibernation. This complex contains four species of proteins, HP-20, -25, -27 and -55. In the present study, cDNA clones coding for the chipmunk HP-55 were isolated from a liver cDNA library. Sequence analysis revealed that HP-55 is produced as a precursor protein of 413 amino acids (aa), that it has a signal peptide of 24 aa, and that it contains four potential N-glycosylation sites. The deduced aa sequence shows 63% identity with that of rat alpha1-antitrypsin (alpha1-AT); however, the sequence corresponding to the reactive center P1-P1' residues was found to be Met-Leu, whereas it is Met-Ser in the rat alpha1-AT. During screening of the chipmunk liver cDNA library, four other related classes of cDNA clones were obtained, each also coding for an alpha1-AT-like protein. In spite of more than 86% overall aa sequence identity among the five chipmunk alpha1-AT-like proteins, they are highly divergent in the putative reactive center region; the putative P1-P1' sequences are Met-Leu (HP-55 or CM55-ML), Met-Met (CM55-MM), Met-Ser (CM55-MS), Ser-Ile (CM55-SI) and Ser-Thr (CM55-ST). Each of the alpha1-AT-...Continue Reading

References

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Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F Borriello, K S Krauter
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Citations

Jul 21, 2000·European Journal of Biochemistry·M KojimaT Shiba
May 1, 2007·Gene·Daisuke TsukamotoNobuhiko Takamatsu
Nov 18, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Katsumi MominokiMasayuki Saito
Apr 18, 2006·Cell·Noriaki KondoTakashi Ohtsu
Nov 17, 2009·Bioorganicheskaia khimiia·L I KramarovaE N Gakhova
Sep 25, 2003·Physiological Reviews·Hannah V CareySandra L Martin
May 17, 2002·Journal of Applied Physiology·Frank Van Breukelen, Sandra L Martin

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