Expression of multiple stem cell markers in dental pulp cells cultured in serum-free media

Journal of Endodontics
Thais Miyuki HirataMarkus Haapasalo

Abstract

Stem cell lines are usually grown in medium containing animal products. Fetal bovine serum (FBS) is an important additive for cell growth; however, the allergenic potential and the possibility of contamination when we use a medium containing serum would be a barrier to transplantation and consequently to the introduction of cell therapy methods into clinical applications. Dental mesenchymal cells were isolated and expanded in vitro and maintained in 4 different serum-free media (SFMs): SFM#1 (ITS-X, embryotrophic factor [ETF]); SFM#2 (ITS-X); SFM#3 (ETF); and SFM#4 (ETF, sodium pyruvate, ascorbic acid, fibroblast growth factor [FGF-a], acidic). Viability, proliferative, and immunocytochemical tests for the cells were performed by using 4 stem cell markers (CD44H, CK19, nestin, and P63) for ectoderm, mesoderm, and endoderm. Viability tests showed a significant difference between the control and SFMs in both deciduous tooth pulp cells (DTPCs) and wisdom tooth pulp cells (WTPCs). However, all SFMs demonstrated 84%-90% viability, whereas the control showed 90%-93%. In both DTPCs and WTPCs, SFM#1 had the highest proliferation rate among the 4 SFMs. Immunocytochemistry stained positive stem cell markers most intensely in cells cultur...Continue Reading

Citations

Sep 4, 2013·Regenerative Medicine·Nikolay IshkitievToshio Imai
Dec 12, 2012·Journal of Endodontics·Pakize Neslihan TaşlıFikrettin Sahin
Mar 15, 2012·Journal of Endodontics·Nikolay IshkitievMarkus Haapasalo
Jul 21, 2015·Journal of Endodontics·Maxime DucretEmeline Perrier-Groult
Apr 15, 2015·Stem Cells and Development·Petra HilkensIvo Lambrichts
Sep 18, 2012·Archives of Oral Biology·Nobuyuki Kawashima
Mar 21, 2017·Tissue Engineering. Part C, Methods·Yusuke TakebeKazuhito Satomura
Mar 14, 2017·Tissue Engineering and Regenerative Medicine·Beatriz A Rodas-Junco, Claudia Villicaña
Nov 16, 2019·Scientific Reports·Kengo NakajimaKotaro Tanimoto
Jan 13, 2015·The Journal of Clinical Pediatric Dentistry·Werle Stefanie BressanLuciano Casagrande
Sep 22, 2020·Nature Reviews. Materials·Elizabeth A Aisenbrey, William L Murphy
Apr 3, 2012·Journal of Cell Science·Maher AtariLuis Giner

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