Expression of Multiple Taenia Solium Immunogens in Plant Cells Through a Ribosomal Skip Mechanism

Molecular Biotechnology
Elizabeth Monreal-EscalanteSergio Rosales-Mendoza

Abstract

Taenia solium cysticercosis is a major parasitic disease that affects the human health and the economy in underdeveloped countries. Porcine cysticercosis, an obligatory stage in the parasite life cycle, is a suitable target for vaccination. While several recombinant and synthetic antigens proved to be effective as vaccines, the cost and logistic difficulties have prevented their massive use. Taking this into account, a novel strategy for developing a multi-epitope low-cost vaccine is herein explored. The S3Pvac vaccine components (KETc1, KETc12, KETc7, and GK1 [KETc7]) and the protective HP6/TSOL18 antigen were expressed in a Helios2A polyprotein system, based on the 'ribosomal skip' mechanism mediated by the 2A sequence (LLNFDLLKLAGDVESNPG-P) derived from the Foot-and-mouth disease virus, which induces self-cleavage events at a translational level. This protein arrangement was expressed in transgenic tobacco cells. The inserted sequence and its transcript were detected in several Helios2A lines, with some lines showing recombinant protein accumulation levels up to 1.3 µg/g of fresh weight in leaf tissues. The plant-derived Helios2A vaccine was recognized by antibodies in the cerebral spinal fluid from neurocysticercosis patien...Continue Reading

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Citations

Sep 22, 2015·Plant Biotechnology Journal·Hui-Ting Chan, Henry Daniell
Jun 16, 2015·Vaccine·Jorge A Salazar-GonzálezSergio Rosales-Mendoza
Sep 1, 2016·Expert Review of Vaccines·Jaime I Arevalo-VillalobosSergio Zarazua
Aug 11, 2018·Reviews in Medical Virology·Garry A Luke, Martin D Ryan
Sep 2, 2020·Molecular Biotechnology·Evelia M Milán-NorisSchuyler S Korban
Aug 9, 2017·Frontiers in Microbiology·Xiaoyao YangXiaoyue Chen
Apr 17, 2020·Vaccines·Sergio Rosales-MendozaJaime I Arévalo-Villalobos
Feb 23, 2021·Expert Review of Vaccines·Abel Ramos-VegaCarlos Angulo

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