Expression of multiple troponin T variants in neonatal chicken breast muscle

Developmental Biology
H AbeT Obinata

Abstract

The types of troponin-T (TNT) expressed in neonatal chicken breast muscle were examined by two-dimensional gel electrophoresis (2-D PAGE), immunoblotting, and peptide mapping. When troponin from neonatal chicken breast muscle or whole lysate of the muscle was displayed on 2-D PAGE, multiple spots were observed in the TNT region on the gel. They differed slightly from those in adult breast- and leg-type TNT, but were positively stained with the antibody specific for TN-T. These results indicate that multiple spots observed in the TNT region are all TNT isoforms. The TNT isoforms in the neonatal breast muscle were classified into two groups, based on size. Each group contained about five variants. The first group with a larger size was in the molecular weight range of adult breast TNT, while the smaller-sized second group was in the molecular weight range of adult leg TNT. Overall peptide map patterns of variants in the first group and also that of adult breast TNT resembled each other, whereas those of variants in the second group were similar to that of adult leg TNT. The TNT of adult breast-type appeared at about 2- to 3-weeks posthatch, and thereafter became a major TNT isoform.

References

Sep 1, 1977·The Journal of Cell Biology·G F Gauthier, S Lowey
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1979·Journal of Biochemistry·I Ohtsuki
Mar 1, 1977·Somatic Cell Genetics·M L GefterM D Scharff
Jan 15, 1985·The Biochemical Journal·V V RisnikN B Gusev
Feb 1, 1971·Journal of Biochemistry·S EbashiF Ebashi
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Oct 1, 1982·The Journal of Cell Biology·F C ReinachD A Fischman
Aug 1, 1983·Analytical Biochemistry·A L De Blas, H M Cherwinski
Sep 1, 1984·Developmental Biology·T ObinataD A Fischman
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R MatsudaR C Strohman
Feb 1, 1981·Developmental Biology·R MatsudaY Shimada
Dec 15, 1971·FEBS Letters·W DrabikowskiB Barylko

❮ Previous
Next ❯

Citations

Apr 1, 1989·Developmental Biology·M NakamuraT Hirabayashi
Dec 1, 1992·Developmental Biology·E Bandman
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S OhshimaT Obinata
Jan 1, 1996·The Journal of Cell Biology·S YoshidaY Nabeshima
Mar 1, 1991·General and Comparative Endocrinology·K YamanoY Inui
Nov 3, 1987·Biochemistry·J LeszykJ H Collins
May 1, 1990·Circulation Research·A Malhotra
Nov 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Heriberto CuellarGraciela A Unguez
Jun 11, 1999·The Journal of Biological Chemistry·E A BucherC P Emerson
Dec 1, 1991·Journal of Muscle Research and Cell Motility·S FujitaY Maéda
Jun 1, 1988·Journal of Muscle Research and Cell Motility·M M BriggsF H Schachat
Feb 1, 1994·Journal of Muscle Research and Cell Motility·Y YaoT Hirabayashi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.