Expression of Mx protein and interferon-alpha in pigs experimentally infected with swine influenza virus

Veterinary Pathology
K Jung, C Chae

Abstract

Expression of Mx protein and interferon-alpha (IFN-alpha) was examined by immunohistochemistry in pigs experimentally infected with swine influenza virus. In infected pigs euthanatized at 1 day postinoculation (dpi), the lumen of bronchioles were filled with large numbers of mononuclear cells, small numbers of neutrophils, sloughing epithelial cells, and proteinaceous fluid. Lesions at 3 and 5 dpi were similar but less severe. Alveolar spaces were filled with neutrophils. By 7 and 10 dpi, microscopic lesions were resolved. The immunohistochemical signals for Mx protein and IFN-alpha antigen were confined to cells in areas that had hybridization signal for swine influenza virus. In situ hybridization and immunohistochemistry of serial sections of lung indicated that areas containing numerous swine influenza virus RNA-positive cells also have numerous Mx and IFN-alpha antigen-positive cells. Mean immunohistochemical scores for Mx protein-positive cells were correlated with mean immunohistochemical scores for IFN-alpha antigen-positive cells (r(s) = 0.8799, P < 0.05). These results indicated that Mx protein and IFN-alpha antigen were expressed in the lung from pigs experimentally infected with swine influenza virus, but their biol...Continue Reading

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Citations

May 19, 2012·Case Reports in Anesthesiology·Ayse B OzerOzden Yildizhan
Feb 20, 2009·Journal of Virology·Hana M WeingartlJürgen A Richt
Aug 30, 2008·Immunogenetics·Sofia BerlinHans Ellegren
Feb 24, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M PalmD Desmecht
Feb 7, 2019·Frontiers in Veterinary Science·Daniela S RajaoDaniel R Perez
Mar 31, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jiangnan HuangMei Yu

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Datasets Mentioned

BETA
M65087
M28623
AF017079

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis

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