Abstract
To identify and compare specific protein levels between overacting inferior oblique (IO) muscles in superior oblique (SO) palsy patients and normal IO muscles. We obtained 20 IO muscle samples from SO palsy patients with IO overaction ≥ + 3 who underwent IO myectomies (IOOA group), and 20 IO samples from brain death donors whose IO had functioned normally, according to their ophthalmological chart review (control group). We used MyoD for identifying satellite cell activation, insulin-like growth factor binding protein 5 (IGFBP5) for IGF effects, thioredoxin for oxidative stress, and p27 for satellite cell activation or oxidative stress in both groups. Using immunohistochemistry and Western blot, we compared expression levels of the four proteins (MyoD, IGFBP5, thioredoxin, and p27). Levels of thioredoxin and p27 were decreased significantly in the IOOA group. MyoD and IGFBP5 levels showed no significant difference between the groups. Based on these findings, the overacting IOs of patients with SO palsy had been under oxidative stress status versus normal IOs. Pathologically overacting extraocular muscles may have an increased risk of oxidative stress compared with normal extraocular muscles.
References
May 1, 1993·Circulation·H ItoF Marumo
Jul 1, 1999·Genes & Development·C J Sherr, J M Roberts
Jul 18, 2001·Journal of Applied Physiology·T J Hawke, D J Garry
Apr 3, 2002·The EMBO Journal·Toru TanakaJunji Yodoi
May 15, 2002·Physiological Genomics·M Dominik FischerTejvir S Khurana
Jun 12, 2003·Ophthalmology·Reika Kono, Joseph L Demer
Jan 14, 2005·Molecular Biology of the Cell·Graziella MessinaMilena Grossi
Apr 1, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jason M HansenDean P Jones
Sep 22, 2006·Antioxidants & Redox Signaling·Dean P Jones
Feb 3, 2007·Muscle & Nerve·Jennifer S Moylan, Michael B Reid
Apr 28, 2009·Journal of AAPOS : the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus·Abbas BagheriShahin Yazdani
Feb 24, 2010·BMC Developmental Biology·Robert B WhitePeter S Zammit
Nov 26, 2010·Nutrition Research and Practice·Lesley QuintosJong-Sang Kim
Dec 17, 2011·Journal of Signal Transduction·Elena Barbieri, Piero Sestili
Sep 10, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Su Kyung JungSun Young Shin
Sep 23, 2014·Developmental Biology·Pascal StuelsatzZipora Yablonka-Reuveni
Apr 30, 2015·Development·Nicolas A DumontMichael A Rudnicki
Sep 30, 2015·Canadian Journal of Physiology and Pharmacology·Thomas F WhayneNilanjana Maulik
Jan 28, 2016·Molecules and Cells·Luis E S Netto, Fernando Antunes