PMID: 9169095Apr 1, 1997Paper

Expression of myogenic regulatory factors in normal and dystrophic mice: effects of IGF-1 treatment

Biochemical and Molecular Medicine
H H HsuP W Speiser

Abstract

Myogenic regulatory factors (MRFs) promote differentiation of muscle cells from fibroblasts and are induced by insulin-like growth factor I (IGF-1). Prior studies have shown synthesis of new muscle protein and improved muscle morphology when mature dy mice with muscular dystrophy are treated with IGF-1. We investigated whether these salutary effects of IGF-1 might be attributable to stimulation of MRFs. Male dy (129ReJ) mice and controls (129J) were assigned to IGF-1 treatment (10 micrograms twice daily) or non-treatment at about 5 weeks of life and sacrificed 6 weeks later. RNA was extracted from skeletal muscles, reverse transcribed, and amplified by polymerase chain reaction (PCR) using primers specific for each MRF. Competitive PCR was performed to quantify MyoD expression in response to IGF-1 treatment. Transcripts for myf-5, MRF4, and myogenin were detected in both control and dy mouse muscles; no apparent differences were observed between treatment groups. Quantitative analysis of transcripts for MyoD indicated no significant basal differences between control and dy mice. There was, however, significantly higher MyoD expression in the dy group, and a trend toward significance in the control group, following IGF-1 treatme...Continue Reading

References

Dec 1, 1989·Genes & Development·S J Rhodes, S F Konieczny
Dec 1, 1986·Experimental Neurology·C R AshmoreY B Lee
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·S M Rosenthal, Z Q Cheng
Nov 1, 1995·Endocrinology·M M ZdanowiczA E Slonim
Jun 30, 1995·Annals of the New York Academy of Sciences·K E DaviesD J Blake
Mar 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M A Rudnicki, R Jaenisch
Dec 1, 1993·Genes & Development·L Powell-BraxtonT A Stewart
Dec 1, 1995·The Journal of Clinical Endocrinology and Metabolism·E VlachopapadopoulouA E Slonim
May 1, 1996·Journal of Neuropathology and Experimental Neurology·S BhagavatiB Leung
May 15, 1996·Genes & Development·L A MegeneyM A Rudnicki
Aug 1, 1996·BioTechniques·F SouazéP Forgez

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Citations

Jun 15, 2011·Cell and Tissue Research·Neill J Turner, Stephen F Badylak
Nov 9, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eric D RabinovskyRobert J Schwartz
Oct 24, 2002·Clinical Orthopaedics and Related Research·Geoffrey GoldspinkHamish Simpson

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