Expression of N-methyl-D-aspartate receptor subunits in the bovine ovum: ova as a potential source of autoantigens causing anti-NMDAR encephalitis

The Tohoku Journal of Experimental Medicine
Naoko TachibanaShu-Ichi Ikeda

Abstract

Autoimmune synaptic encephalitis is characterized by the presence of autoantibodies against synaptic constituent receptors and manifests as neurological and psychiatric disorders. Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is such an autoimmune disorder that predominantly affects young women. It is associated with antibodies against the extracellular region of the NR1 subunit of postsynaptic NMDAR. Each NMDAR functions as a heterotetrameric complex that is composed of four subunits, including NR1 and NR2A, NR2B, or NR2C. Importantly, ovarian teratoma is a typical complication of anti-NMDAR encephalitis in female patients and may contain antigenic neural tissue; however, antigenic sites remain unknown in female patients without ovarian teratoma. The purpose of this study was to investigate the expression of NMDARs in the ovum. We detected NR1 and NR2B immunoreactivity in protein fractions extracted from the bovine ovary and ova by SDS-polyacrylamide gel electrophoresis and immunoblotting analysis. Immunoprecipitates digested with trypsin were analyzed by reverse phase liquid chromatography coupled to tandem mass spectrometry. We obtained the following five peptides: SPFGRFK and KNLQDR, which are consistent with part...Continue Reading

References

Jul 2, 1992·Nature·T KutsuwadaM Arakawa
Jun 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiK L Kalra
Jun 12, 2001·Current Opinion in Neurobiology·S Cull-CandyM Farrant
Jul 18, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiC R Taylor
Jan 24, 2003·Annual Review of Pharmacology and Toxicology·Robert J WentholdRonald S Petralia
Aug 20, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Manabu AbeKenji Sakimura
Sep 24, 2005·Annals of Neurology·Roberta VitalianiJosep Dalmau
Nov 11, 2005·Nature·Hiroyasu FurukawaEric Gouaux
Sep 19, 2006·Biochemical and Biophysical Research Communications·Hideaki Kume, Fuyuki Kametani
Nov 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Laurent GrocDaniel Choquet
May 5, 2007·Nature Clinical Practice. Neurology·Lauren H SansingJosep Dalmau
Nov 23, 2007·Journal of Neurology, Neurosurgery, and Psychiatry·M SekiJ Dalmau
Feb 27, 2008·Neurology·María Elena Novillo-LópezJaime Masjuan
Aug 19, 2008·Journal of Neurology, Neurosurgery, and Psychiatry·A EkerB Elibol
Apr 28, 2009·Genome Biology·Aleksey V ZiminSteven L Salzberg
Aug 12, 2009·Annals of Neurology·Nicole R FloranceJosep Dalmau
Apr 30, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ethan G HughesRita J Balice-Gordon
Dec 18, 2010·Lancet Neurology·Josep DalmauRita Balice-Gordon
Feb 18, 2011·Case Reports in Neurology·Kana TojoShu-Ichi Ikeda
Jun 11, 2011·The Journal of Biological Chemistry·Jing-yuan CaoJian-hong Luo
Jul 13, 2011·Neurology·Eric LancasterJosep Dalmau
May 1, 2012·Brain : a Journal of Neurology·Lenka MikasovaLaurent Groc
Aug 10, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amy J GleichmanDavid R Lynch
Jul 25, 2013·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Fuyuki Kametani, Shu-ichi Ikeda

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