PMID: 7515582May 1, 1994Paper

Expression of Na-P(i) cotransport in rat kidney: localization by RT-PCR and immunohistochemistry

The American Journal of Physiology
M CusterB Kaissling

Abstract

We have recently identified a rat kidney cortex Na-dependent transport system for phosphate (P(i)) by expression cloning (NaP(i)-2) (S. Magagnin, A. Werner, D. Markovich, V. Sorribas, G. Stange, J. Biber, and H. Murer. Proc. Natl. Acad. Sci. USA 90: 5979, 1993). In this study we have used reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry to establish the sites of expression of the NaP(i)-2-related mRNA and protein. RT-PCR was performed with single microdissected nephron segments. From these experiments we conclude that NaP(i)-2 mRNA is predominantly expressed in the proximal tubules of superficial and deep nephrons. No NaP(i)-2 mRNA was detected in the thick ascending limb of Henle's loop; however, faint NaP(i)-2 related PCR products were also observed in collecting ducts. Expression of the NaP(i)-2 protein was examined with the use of polyclonal antibodies raised against synthetic NaP(i)-2-derived peptides. Strong specific anti-NaP(i)-2 antiserum-mediated immunofluorescence was found in the convoluted part of proximal tubules and gradually decreased along the straight part. Immunofluorescence indicated that the NaP(i)-2 protein is present in the brush border of proximal tubular cells. In additio...Continue Reading

Citations

Feb 8, 2000·American Journal of Physiology. Renal Physiology·M TraebertB Kaissling
Mar 7, 2002·American Journal of Physiology. Renal Physiology·Katja KöhlerHeini Murer
Jan 9, 2003·Annual Review of Physiology·Heini MurerJürg Biber
Dec 6, 2003·American Journal of Physiology. Renal Physiology·Harriet S TenenhouseRené St-Arnaud
Jul 22, 2004·American Journal of Physiology. Renal Physiology·Li E YangAlicia A McDonough
Mar 7, 2008·American Journal of Physiology. Renal Physiology·Carsten A WagnerJohannes Loffing
Aug 15, 2008·American Journal of Physiology. Renal Physiology·Julia ThumfartDominik Müller
Dec 17, 2008·American Journal of Physiology. Renal Physiology·Ricardo Villa-BellostaIan C Forster
Jun 11, 2010·American Journal of Physiology. Renal Physiology·Joanne MarksRobert J Unwin
Jan 8, 2019·Pflügers Archiv : European journal of physiology·Moshe Levi, Enrico Gratton
Aug 6, 2020·International Journal of Molecular Sciences·Giovanni LombardiSabrina Corbetta
Mar 11, 2000·American Journal of Physiology. Renal Physiology·C ShachafM Tzukerman
Mar 11, 2000·American Journal of Physiology. Renal Physiology·N HernandoH Murer
Dec 18, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Paul L DudasJ Larry Renfro
Oct 4, 2000·Physiological Reviews·H MurerJ Biber
Jun 19, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Patrick K K LeongAlicia A McDonough
Mar 5, 2004·American Journal of Physiology. Renal Physiology·Craig B WodaSusan E Mulroney
Apr 11, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C WodaA Haramati
Aug 5, 2000·American Journal of Physiology. Renal Physiology·C E MagyarA A McDonough
Nov 13, 2001·American Journal of Physiology. Renal Physiology·Y SoumounouH S Tenenhouse
May 12, 2000·American Journal of Physiology. Renal Physiology·M TraebertB Kaissling
Mar 25, 2005·American Journal of Physiology. Cell Physiology·Nadine DéliotHeini Murer
Sep 4, 2003·American Journal of Physiology. Renal Physiology·Harriet S TenenhouseKen-ichi Miyamoto
Jul 3, 2003·American Journal of Physiology. Renal Physiology·Sandra PribanicHeini Murer
Feb 20, 2009·American Journal of Physiology. Renal Physiology·Sonja C ReiningNati Hernando
May 21, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Pedro M GuerreiroJ Larry Renfro
Dec 19, 2002·American Journal of Physiology. Renal Physiology·C GrahamA Werner
Feb 11, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tamara RadanovicJürg Biber
Feb 24, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·A Werner, R K Kinne
Jan 22, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Arezoo DaryadelIsabel Rubio-Aliaga
Apr 23, 2013·EMBO Molecular Medicine·Joachim AlbersIan J Frew
Dec 8, 2020·American Journal of Physiology. Renal Physiology·Mathieu Lemaire
Jun 13, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Elisa De PaolisEttore Capoluongo
Mar 20, 2021·Journal of Cellular Physiology·Tomasz Kulesza, Agnieszka Piwkowska

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