Expression of NADPH oxidase is induced by all-trans retinoic acid but not by phorbol myristate acetate and 1,25 dihydroxyvitamin D3 in the human promyelocytic cell line NB4

Leukemia
E N N'DiayeI Maridonneau-Parini

Abstract

Human promyelocytic cells, NB4, differentiate into neutrophils in response to all-trans retinoic acid (ATRA). It has recently been proposed that NB4 cells have bilineage potential because these cells are also able to differentiate into monocyte/macrophages when exposed to a combination of 1,25-dihydroxyvitamin D3 (VD3) and phorbol myristate acetate (PMA). Differentiation of myeloid cells into neutrophils or monocytes is associated with the acquisition of the O2- producing enzyme, NADPH oxidase, which plays a critical role in microbial killing. In this study, the expression of the components of the NADPH oxidase complex during the differentiation of NB4 cells into neutrophils or macrophages has been investigated. Whereas cells exposed to ATRA were able to produce O2- after 2 days of differentiation, they remain unable to generate O2- when exposed to PMA or PMA + VD3. With the exception of p21rac, none of the other oxidase components was expressed in non-differentiated cells. Addition of ATRA induced the progressive expression and accumulation of p22phox, p91phox, p47phox and p67phox. Compared to the other components, p67phox was expressed late and its expression appeared to correlate most closely with the generation of O2- in th...Continue Reading

Citations

Apr 3, 1998·Journal of Cellular Physiology·C GrégoireI Maridonneau-Parini
Sep 13, 2014·Cytokine & Growth Factor Reviews·Jenish R Patel, Adolfo García-Sastre
Sep 2, 2005·Veterinary Immunology and Immunopathology·A Gruden-Movsesijan, Lj Sofronic Milosavljevic
Feb 6, 2014·The British Journal of Nutrition·Siyuan ChenWeiping Wang
Aug 4, 2011·PloS One·Kaitlin J SoyeWilson H Miller
Jan 12, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Claus KerkhoffJacques Doussiere
Jun 1, 2018·Oxidative Medicine and Cellular Longevity·Milica VelimirovićNataša D Petronijević
Jan 29, 2021·Journal of Inflammation Research·Marfa BlanterSofie Struyf
Oct 3, 1999·Antimicrobial Agents and Chemotherapy·C Miossec-BartoliA Diu-Hercend

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