Expression of Na+/HCO3- co-transporter proteins (NBCs) in rat and human skeletal muscle

Acta Physiologica Scandinavica
J M KristensenC Juel

Abstract

Sodium/bicarbonate co-transport (NBC) has been suggested to have a role in muscle pH regulation. We investigated the presence of NBC proteins in rat and human muscle samples and the fibre type distribution of the identified NBCs. Western blotting of muscle homogenates and sarcolemmal membranes (sarcolemmal giant vesicles) were used to screen for the presence of NBCs. Immunohistochemistry was used for the subcellular localization. The functional test revealed that approximately half of the pH recovery in sarcolemmal vesicles produced from rat muscle is mediated by bicarbonate-dependent transport. This indicates that the NBCs are preserved in the vesicles. The western blotting experiments demonstrated the existence of at least two NBC proteins in skeletal muscle. One NBC protein (approximately 150 kDa) seems to be related to the kidney/pancreas/heart isoform NBC1, whereas the other protein (approximately 200 kDa) is related to the NBC4 isoform. The two NBC proteins represent the electrogenic isoforms named NBCe1 and NBCe2. Membrane fractionation and immunofluorescence techniques confirmed that the two NBCs are located in the sarcolemmal membrane as well as in some internal membranes, probably the T-tubules. The two NBCs localized...Continue Reading

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Citations

Feb 4, 2006·European Journal of Applied Physiology·Carsten Juel
Apr 8, 2006·Pflügers Archiv : European journal of physiology·Matthew D BeekleyGerolf Gros
Aug 7, 2013·Current Opinion in Nephrology and Hypertension·Ira Kurtz, Quansheng Zhu
Oct 8, 2011·Journal of the American Society of Nephrology : JASN·Peter S Aronson, Gerhard Giebisch
Mar 22, 2016·Journal of Aging Research·Gina RutherfordJulia L Newton
Aug 25, 2015·European Journal of Applied Physiology·Claire ThomasChristine Hanon
Jul 22, 2006·Biochimica Et Biophysica Acta·James L KreindlerRobert J Bridges
Dec 15, 2017·The Journal of General Physiology·Christian Aalkjær, Ole Bækgaard Nielsen
Aug 30, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Helle Hasager DamkierJeppe Praetorius
Feb 8, 2006·American Journal of Physiology. Renal Physiology·Alexander Pushkin, Ira Kurtz
Apr 17, 2013·Physiological Reviews·Mark D Parker, Walter F Boron
Jul 5, 2007·American Journal of Physiology. Endocrinology and Metabolism·Claire ThomasJacques Mercier
Mar 27, 2010·Journal of Applied Physiology·George A Brooks
Sep 27, 2020·Nutrients·Dheeraj Kumar Posa, Shahid P Baba
Oct 21, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Claire ThomasGeorge A Brooks
Mar 18, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yoshinori TanakaYutaka Kano
Oct 9, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·John J GildeaRobin A Felder

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