Expression of nerve growth factor and its receptors in the uterus of gilts with endometritis induced by infection with Escherichia coli

Journal of Comparative Pathology
Barbara Jana, Aneta Andronowska

Abstract

Nerve growth factor (NGF) is a neurotrophin implicated in the pathophysiology of allergy, diseases of the immune system and inflammation. Expression of NGF and its receptors (TrkA and p75) has not been examined in inflamed uterine tissue. The aim of the present study was to evaluate the patterns of NGF, TrkA and p75 expression in normal and inflamed porcine uteri using real-time reverse transcriptase polymerase chain reaction (RT-PCR), western blotting and immunohistochemistry. On day 3 of the oestrous cycle, 50 ml of saline or 50 ml of Escherichia coli suspension containing 10(9) colony forming units/ml, was injected into both uterine horns of control (n = 10) and experimental (n = 10) gilts, respectively. Infected animals developed moderate or severe acute endometritis after 8 days and moderate acute or subacute endometritis after 16 days. On day 8 of the study, the expression of NGF and TrkA mRNA and protein was higher in the endometrium of gilts with endometritis than in normal tissue. NGF protein was also more prominently expressed in the endometrium of those animals with endometritis 16 days post infection. There was no difference in endometrial p75 mRNA and protein expression on either day. The myometrium of animals in b...Continue Reading

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Citations

Jul 21, 2016·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Hongyi TanXiaoli Su
Aug 9, 2013·Reproduction, Fertility, and Development·Barbara JanaJerzy Jaroszewski
Feb 9, 2021·Frontiers in Veterinary Science·Daniela E BarrazaMartin E Argañaraz

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