PMID: 16536986Mar 16, 2006Paper

Expression of NET-1 gene and protein in hepatocellular carcinoma and related tissues

Ai zheng = Aizheng = Chinese journal of cancer
Li ChenXing-Yu Li

Abstract

NET-1 is a new tumor-related gene. So far, its expression in hepatocellular carcinoma (HCC) hasn't been reported. This study was to screen and compare the expression of NET-1 among fetal liver tissue, adult normal liver tissue, HCC and adjacent noncancerous tissues; explore its expression feature in HCC. The expression of NET-1 mRNA in 3 specimens of fetal liver tissue, 4 specimens of adult liver tissue, 4 specimens of test HCC tissue, and 28 specimens of HCC and adjacent noncancerous tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression level of NET-1 gene was analyzed by Four-Star image analysis software. NET-1 gene polyclonal antibody was generated by gene biological engineering, and its expression in human hepatic cancer cell line SMMC-7721 was observed under laser confocal microscope. The expression of NET-1 protein in the 28 specimens of HCC and adjacent noncancerous tissues was detected by immunohistochemistry. NET-1 mRNA was expressed in all of the 4 specimens of test HCC tissue, but didn't express in normal adult and fetal liver tissues; the positive rates of NET-1 mRNA were 85.71% (24/28) in both HCC and adjacent noncancerous tissues. The mRNA level of NET-1 was significantly...Continue Reading

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