Expression of neuronal nicotinic acetylcholine receptors in rat vagal pulmonary sensory neurons.

Respiratory Physiology & Neurobiology
Qihai GuLu-Yuan Lee

Abstract

It is known that cigarette smoke inhalation causes airway irritation and cough, and the effect is caused by both direct and indirect stimulatory effects of nicotine on bronchopulmonary sensory nerves. However, little is known about the expression of nicotinic acetylcholine receptors (nAChRs) in these afferents. In the present study, whole-cell patch-clamp recording and RT-PCR were carried out to examine the expression and function of nAChRs in isolated rat vagal pulmonary sensory neurons that were identified by retrograde labeling with a fluorescent tracer. Patch-clamp recordings demonstrated that application of acetylcholine concentration-dependently evoked an inward current in a subset of pulmonary sensory neurons, which was inhibited by hexamethonium. Application of nicotine or 1,1-dimethyl-4-phenylpiperazinium (DMPP) also activated these neurons, evoking an inward current in voltage-clamp configuration and causing depolarization and action potential in current-clamp recordings. RT-PCR analysis further demonstrated the expression of mRNA encoding for the nAChR subunits alpha4, alpha5, alpha6, alpha7, beta2, beta3 and beta4, but not alpha2 and alpha3 in these neurons.

References

Nov 19, 1997·Progress in Neurobiology·C GottiF Clementi
Sep 18, 2002·Journal of Applied Physiology·Kevin Kwong, Lu-Yuan Lee
Jan 15, 2005·Progress in Neurobiology·C Gotti, F Clementi
Jul 22, 2005·Journal of Medicinal Chemistry·Anders A JensenPovl Krogsgaard-Larsen
Jul 27, 2007·The FEBS Journal·Dimitra KalamidaSocrates J Tzartos

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Citations

Jun 21, 2008·Histochemistry and Cell Biology·W KummerU Pfeil
Jul 20, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Youmin HuLu-Yuan Lee
Jun 10, 2011·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Magalie Lenoir, Eugene A Kiyatkin
Oct 10, 2009·Current Allergy and Asthma Reports·Kirsten C VerheinDavid B Jacoby
Jul 1, 2008·Respiratory Physiology & Neurobiology·Lu-Yuan Lee
Dec 31, 2008·Journal of Neuroscience Research·Ozra DehkordiAnnapurni Jayam-Trouth
Jun 8, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M HübschM Raab
Sep 17, 2014·Journal of Neuroscience Research·Ozra DehkordiAnnapurni Jayam-Trouth
Jun 1, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·M KhosraviL-Y Lee
Sep 27, 2019·Frontiers in Physiology·Alexandria K Driessen
May 19, 2021·Archives of Biochemistry and Biophysics·Niyati A BorkarChristina M Pabelick

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