PMID: 2493000Feb 1, 1989Paper

Expression of NF-L and NF-M in fibroblasts reveals coassembly of neurofilament and vimentin subunits

The Journal of Cell Biology
M J Monteiro, D W Cleveland

Abstract

We have used transient and stable DNA transfection to force synthesis of the mouse NF-L and NF-M genes in nonneuronal cultured animal cells. When the authentic NF-L gene (containing 1.7 kb of sequences 5' to the transcription initiation site) was transfected into L cells, correctly initiated NF-L mRNA was produced from the transfected gene but not the endogenous NF-L genes. Therefore, the normal restriction of NF-L expression to neurons cannot derive exclusively from absence in nonneuronal cells of neuron-specific transcription factors. When the NF-L coding region was linked to the strong promoter from Moloney Murine Sarcoma virus, we obtained high levels of synthesis of NF-L subunits (accumulating to as much as 9% of cell protein in stable cell lines). Although NF-L and NF-M polypeptides are normally expressed exclusively in postmitotic neurons, NF-L or NF-M polypeptides expressed in fibroblasts were efficiently assembled into intermediate filament arrays, thus demonstrating the competence of both NF-L and NF-M to assemble in vivo in the absence of additional neuron-specific factors. As judged by immunofluorescence localization and by the alteration in the solubility of the endogenous vimentin filaments, filaments containing N...Continue Reading

References

Nov 25, 1979·Journal of Molecular Biology·D C RuegerA Bignami
Dec 1, 1978·The Journal of Cell Biology·R K LiemM L Shelanski
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W W FrankeK Weber
Dec 15, 1976·Journal of Molecular Biology·P M SteinertS B Zimmerman
Nov 20, 1986·Journal of Molecular Biology·R A QuinlanJ Engel
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·V M LeeR A Lazzarini
Jan 1, 1988·The Journal of Cell Biology·D G LeonardE B Ziff
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Aug 21, 1985·Biochimica Et Biophysica Acta·J P JulienF Grosveld
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Dec 17, 1971·Science·M L ShelanskiW T Norton
Sep 1, 1968·The Journal of Cell Biology·H IshikawaH Holtzer
Aug 1, 1981·Cell·D W ClevelandM W Kirschner
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L A LaiminsP Gruss
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E WangR K Liem
Jan 1, 1983·Developmental Neuroscience·M M PortierF Gros
Jan 1, 1984·Journal of Neuroscience Research·E E GardnerA Bignami
Apr 1, 1984·The Journal of Cell Biology·N HirokawaM B Willard
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L A Sternberger, N H Sternberger
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P M SteinertR D Goldman
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R A Quinlan, W W Franke
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B Geiger, S J Singer
Jun 25, 1981·Journal of Molecular Biology·W RennerE Mandelkow

❮ Previous
Next ❯

Citations

Sep 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R L Shoeman, P Traub
Jan 1, 1991·Cell Motility and the Cytoskeleton·O Skalli, R D Goldman
Jan 1, 1992·Cell Motility and the Cytoskeleton·R A Nixon, T B Shea
Sep 1, 1991·Journal of Neuroscience Research·D SoiferB Poulos
Sep 1, 1991·Journal of Neuroscience Research·L M ParysekC A Ley
Nov 1, 1991·Journal of Neuroscience Research·M P KaplanR K Liem
Mar 1, 1992·Brain Research. Molecular Brain Research·P S ShneidmanW W Schlaepfer
Sep 11, 1992·Brain Research. Molecular Brain Research·G A ElderR A Lazzarini
Sep 1, 1992·Brain Research. Molecular Brain Research·G A ElderR A Lazzarini
Jan 1, 1993·Brain Research. Molecular Brain Research·S TsuneishiH Nakamura
Apr 1, 1993·Brain Research. Molecular Brain Research·L BeaudetJ P Julien
Sep 1, 1995·Brain Research. Molecular Brain Research·C HsuM J Monteiro
Feb 1, 1992·Current Opinion in Cell Biology·J E ErikssonR D Goldman
Feb 1, 1992·Current Opinion in Cell Biology·R G Oshima
Mar 17, 2004·Biochemical and Biophysical Research Communications·Michael J StrongWei-Wen Ge
Jun 5, 2003·Pharmacology & Therapeutics·Michael J Strong
Oct 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·L J BoyneT B Shea
Nov 1, 1990·The Journal of Cell Biology·J M RaatsH Bloemendal
Jul 1, 1992·The Journal of Cell Biology·B M KellyP J Brophy
Apr 1, 1993·The Journal of Cell Biology·S OkabeN Hirokawa
Sep 1, 1993·The Journal of Cell Biology·M K LeeD W Cleveland
Oct 1, 1994·The Journal of Cell Biology·S TakedaN Hirokawa
Dec 1, 1994·The Journal of Cell Biology·M J MonteiroS Janicki
Feb 11, 1993·Nucleic Acids Research·K YazdanbakhshM Lindenbaum
Feb 17, 2009·Biochemistry. Biokhimii︠a︡·A A Minin, M V Moldaver
Jun 23, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Aidong YuanRalph A Nixon
Nov 1, 1995·Journal of Neurogenetics·R M Twyman, E A Jones
Jun 1, 1993·European Journal of Biochemistry·F A van de KlundertH Bloemendal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.