PMID: 9635256Jun 23, 1998Paper

Expression of nitric oxide synthase isoforms in normal human tracheobronchial epithelial cells in vitro: dependence on retinoic acid and the state of differentiation

Experimental Lung Research
D NorfordP Nettesheim

Abstract

The retinoic acid (RA) and differentiation dependence of constitutive expression of the nitric oxide synthase (NOS) isoforms, iNOS, eNOS, and bNOS, was examined by reverse transcriptase polymerase chain recitation (RT-PCR) in cultured, normal, human, tracheobronchial epithelial (NHTBE) cells. In the presence of RA (RA+), early passage NHTBE cells grown in air-liquid interface (ALI) cultures undergo mucous differentiation; in the absence of RA (RA-), they undergo metaplastic squamous differentiation. Under both conditions the respective differentiated phenotype develops around day 10 of culture. We found that iNOS mRNA levels were much higher in RA+ cultures, expressing the mucous phenotype, than in RA- cultures, expressing the metaplastic squamous phenotype. In contrast, eNOS mRNA levels were much higher in RA- cultures than in RA+ cultures. Expression of bNOS was not significantly affected by the RA status. The pattern of expression of NOS isoforms was then studied during the course of development of the two cellular phenotypes. During the early stages of differentiation, expression of iNOS (RA+) and eNOS (RA-) was very low, indicating that the expression of these two isoforms was not only dependent on the presence or absence ...Continue Reading

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Citations

Apr 15, 2009·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Skyla T CarneyDerek C Norford
Mar 10, 2007·American Journal of Respiratory Cell and Molecular Biology·Vinod SureshSteven C George
Mar 2, 2012·BMC Genomics·Neil R HackettRonald G Crystal
Jul 26, 2006·Free Radical Biology & Medicine·Peter F Bove, Albert van der Vliet
Jan 31, 2018·In Vitro Cellular & Developmental Biology. Animal·Johnny L CarsonMilan J Hazucha
Mar 17, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tadashi KikuchiDaniel J Tschumperlin
Mar 7, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·D V PechkovskyJ Müller-Quernheim

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