Abstract
Previously, our laboratory purified and isolated the cDNA for OBCAM (opioid binding cell adhesion molecule) from bovine brain, as well as highly homologous rat brain cDNA clones, SG13 and DUZ-1. Structural similarities with members of the immunoglobulin superfamily suggest a possible role for OBCAM in cell adhesion and recognition, while studies in our own laboratory suggest that OBCAM is important in the regulation of opioid binding and signal transduction. However, OBCAM lacks a putative transmembrane domain, and its possible mode of linkage to the cellular membrane has not been studied. Upon transfection of Cos 1 cells with SG13 and DUZ-1 cDNAs, the OBCAM-homologous proteins were expressed on the surface of the Cos 1 cells. These proteins were released from the membrane of the Cos 1 cells upon digestion with phosphatidylinositol-specific phospholipase C (PI-PLC), demonstrating that they are linked to the membrane via a phosphatidylinositol (PI) linkage. These results are consistent with a role for OBCAM in cell recognition and adhesion, as well as in cellular signaling.
References
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M LaneN M Lee
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B L KiefferC G Hirth
Dec 28, 1992·Science·C J EvansR H Edwards
Aug 15, 1992·Gene·D A LippmanH H Loh
May 11, 1992·The American Journal of Medicine·D K Owens, R F Nease
Jan 15, 1988·Biochemical and Biophysical Research Communications·S RoyH H Loh
Jan 1, 1988·Annual Review of Immunology·N E Sibinga, A Goldstein
Oct 1, 1987·Journal of Neurochemistry·J HasegawaN M Lee
Jan 1, 1985·Biochemical Pharmacology·P Y LawH H Loh
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T M ChoH H Loh
Jan 1, 1988·Annual Review of Biochemistry·M A Ferguson, A F Williams
Jan 15, 1988·Science·M G Low, A R Saltiel
Jul 29, 1988·Biochemical and Biophysical Research Communications·S RoyN M Lee
Jan 30, 1987·Cell·G A Cross
Dec 1, 1993·Trends in Neurosciences·T Reisine, G I Bell
Feb 1, 1993·The Journal of Cell Biology·K KodukulaS Udenfriend
Nov 1, 1992·Current Biology : CB·N M Hooper
Citations
Jul 11, 2000·The Journal of Comparative Neurology·S MiyataS Maekawa
May 16, 2012·Cancer Discovery·Sherry Y Wu, Anil K Sood
Nov 14, 2013·The FEBS Journal·Sonia MelinoBibudhendra Sarkar
Aug 1, 1997·The Journal of Biological Chemistry·G FanC J Steer
Mar 13, 1999·The Journal of Biological Chemistry·N FunatsuS Maekawa