Expression of organ-specific structures and functions in long-term cultures of aggregates from adult rat liver cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M JuilleratY Guigoz

Abstract

Freshly isolated hepatocytes, endothelial cells, Kupffer and fat-storing cells from adult rats were found to be able to form spheroidal aggregates within 24 hr when cultured under serum-free and rotatory conditions. The ultrastructure, including intracellular organization and extracellular matrix deposition was investigated in 7-day aggregates by scanning and electron microscopy. The different cell types expressed a histotypic organization and reconstructed their extracellular matrix (fibronectin and laminin) as well as junctional complexes (desmosomes, tight junctions and gap junctions). The aggregates preserved, over a period of at least 7 days, high albumin expression (mRNA) and secretion as well as high secretion of the acute phase protein T-kininogen. Dexamethasone (10(-7) M) increased the tyrosine aminotransferase activity fourfold after a 24-hr exposure. Aggregates cultured in the presence of 10(-7) M dexamethasone showed an increased expression and secretion of albumin concomitantly with a decrease in T-kininogen secretion. Induction of 7-ethoxycoumarin O-deethylase (ECOD) was effective after exposure to 3-methylcholanthrene or Aroclor for 48 hr. Up to a 13-fold increase in (ECOD) activity was found. The results demonst...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A E SiricaH C Pitot
Jan 1, 1976·Methods in Cell Biology·P O Seglen
Nov 15, 1976·Experientia·D A Young
Jan 1, 1978·Methods in Enzymology·P MoldéusS Orrenius
Jan 1, 1990·Methods in Enzymology·H F HendriksD L Knook
Jan 1, 1990·Methods in Enzymology·R Blomhoff, T Berg
Feb 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W J LindbladP S Guzelian
Mar 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Blomhoff, K Wake
Mar 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D M BissellF J Roll
Jul 1, 1990·Experimental Cell Research·J Z TongF Alvarez
Jun 1, 1985·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·N MarceauI Leroux-Nicollet
Sep 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F GouletO Morin
Apr 12, 1989·Biochimica Et Biophysica Acta·H Bloemendal, F R Pieper
Sep 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J J Maher
Jul 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S SchäferA M Gressner
Mar 1, 1988·Journal of Cellular Physiology·E G SchuetzP S Guzelian
Jul 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B ClémentA Guillouzo
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Jul 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y YokoiK Usui
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
May 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B ClementA Guillouzo
Jan 1, 1984·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·J Landry, J P Freyer
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R EnatL M Reid
Jan 1, 1983·Molecular and Cellular Biochemistry·C Guguen-Guillouzo, A Guillouzo
Dec 1, 1980·Cell·D Barnes, G Sato
Jan 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·B SaadP Maier
Aug 1, 1993·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·R A Roberts, A R Soames

❮ Previous
Next ❯

Citations

Mar 30, 1999·European Journal of Pharmacology·N MilosevicP Maier
May 4, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C DilworthJ A Timbrell
Aug 30, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T M WalkerC Westmoreland
Nov 8, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T M Walker, A J Woodrooffe
Feb 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P Ammann, P Maier
Dec 14, 2006·In Vitro Cellular & Developmental Biology. Animal·Petra-Maja PrelovsekBoris Bulog
Mar 13, 2015·Current Treatment Options in Oncology·Noriaki Yoshida, Dai Chihara
Jul 1, 1999·Alternatives to Laboratory Animals : ATLA·S CoeckeC Westmoreland

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.