Expression of oxytocin receptor in human pregnant myometrium

Endocrinology
T KimuraF Saji

Abstract

Around the onset of labor, uterine sensitivity to oxytocin (OT) increases tremendously. Although this is considered to reflect OT receptor (OTR) augmentation in myometrium, neither spatial expression of OTR nor the level of the receptor message during the course of pregnancy have been investigated at the molecular level. We examined the localization and expression of the OTR in human myometrium by means of in situ hybridization, immunohistochemistry, and Northern and Western blotting. In the term pregnant myometrium, OTR expressing smooth muscle cells are observed diffusely and heterogeneously. Some of the smooth muscle cells were expressed high levels of the receptor at the messenger RNA and protein level, and they were surrounded with cells weakly positive for the OTR or negative. The level of OTR transcripts increased according to the course of pregnancy. The receptor messenger RNA level reached over 300-fold at parturition compared with the nonpregnant myometrium. In the myometrium at 32 weeks of gestation and not in labor, a relatively large amount (about 100-fold) of the receptor message was expressed. In the nonpregnant myometrium, significant amount of the receptor protein was revealed by Western blotting. We also found...Continue Reading

Citations

Jan 5, 2001·British Journal of Pharmacology·Y ShintaniH Kanaide
Nov 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Masanori OnoYumi Matsuzaki
May 15, 1997·The Journal of Clinical Investigation·R K BansalR K Riemer
Aug 21, 2012·Molecular Endocrinology·Jason N A PontAndrés López Bernal
Dec 31, 2010·Indian Journal of Anaesthesia·D Devikarani, Ss Harsoor
Mar 17, 2004·Reproductive Toxicology·Gary J ChellmanTorsten M Reinheimer
Feb 11, 2005·Journal of Physiology and Biochemistry·A GonzálezN López-Moratalla
Jan 26, 2010·Medicinal Research Reviews·Carlo Mustazza, Giuditta Bastanzio
Apr 25, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiao-Shan MinShi Chang
May 24, 2005·Archives of Gynecology and Obstetrics·Oliver N RichterJ Schmolling
Sep 8, 2004·Journal of the Society for Gynecologic Investigation·Piotr PierzynskiTadeusz Laudanski
Jul 29, 2011·Reproductive Sciences·Melvyn S SoloffSarah K England
Mar 11, 2004·BJOG : an International Journal of Obstetrics and Gynaecology·Etty Daniel-SpiegelEliezer Shalev
Jan 13, 2018·Journal of Neuroendocrinology·Rachael A AugustineColin H Brown
Feb 28, 2003·Biology of Reproduction·Makoto EgawaHideharu Kanzaki
Feb 27, 2003·Biology of Reproduction·Ralph TelgmannRichard Ivell
Jul 24, 2002·Biology of Reproduction·Jennifer CondonWilliam E Rainey
Nov 2, 2016·Endocrinology·Angela YuliaMark R Johnson
Aug 4, 2011·Endocrinology·Victoria Scott, Colin H Brown
May 19, 2006·Expert Opinion on Therapeutic Targets·Mats Akerlund
Aug 18, 2006·Biology of Reproduction·Torsten M ReinheimerWalter H Bee
Aug 4, 2006·Clinical Obstetrics and Gynecology·Jennifer G Smith, David C Merrill
Mar 31, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gerald P McCaffertyTimothy D Westfall
Dec 21, 2007·Biology of Reproduction·Kalidou NdiayeJoy L Pate
Jan 30, 2009·Journal of Perinatology : Official Journal of the California Perinatal Association·K KüblerW M Merz
Jul 8, 2011·Biology of Reproduction·Shirin KhanjaniPhillip R Bennett
Mar 22, 2014·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Filiz Halıcı ÖztürkÖmer Kandemir
Jun 4, 2014·Journal of Neuroendocrinology·S Arrowsmith, S Wray
Jun 21, 2014·Reproduction in Domestic Animals = Zuchthygiene·A GramM P Kowalewski
Feb 13, 2020·Reproductive Sciences·Marina IlicicJonathan W Paul

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