Expression of p-Akt and COX-2 in gastric adenocarcinomas and adenovirus mediated Akt1 and COX-2 ShRNA suppresses SGC-7901 gastric adenocarcinoma and U251 glioma cell growth in vitro and in vivo

Technology in Cancer Research & Treatment
Jing ZhangChunsheng Kang

Abstract

Cyclooxygenase-2 (COX-2) and Protein kinase B (PKB/Akt) play a crucial role in the formation of many malignant tumors and have been shown to be the important therapeutic targets. In the present study, we examined immunohistochemical expression of phosphorylated Akt (p-Akt) and COX-2 in 45 gastric adenocarcinomas with different tumor grades. Then, adenovirus-mediated small hairpin RNA (shRNA) expression vectors rAd5-Akt1+COX-2 (rAd5-A+C) that target sequences of human COX-2 and Akt1 were used to examine the inhibitory effects on cell proliferation, invasion and apoptosis in SGC7901 gastric adenocarcinoma and U251 glioma cells. Cell growth was inhibited by over 70%, as indicated by a MTT assay, and was accompanied by G1/G0 phase arrest in the rAd5-A+C treated group, indicating poor cell growth activities. The number of cells invading through the matrigel in the rAd5-A+C treated group was significantly decreased (36.2+/-3.1) compared with that of the control group SGC7901 (105.0+/-4.0) and the nonsense sequence group rAd5-HK (102.5+/-6.4). In addition, the tumor volumes in the SGC7901 subcutaneous nude mouse model treated with rAd5-A+C was significantly smaller than those of the control group and nonsense sequence group rAd5-HK. W...Continue Reading

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Citations

Apr 30, 2015·International Journal of Immunopathology and Pharmacology·Ya LiBin Ye
Jul 3, 2015·Molecular BioSystems·Meenakshi MalhotraCaitriona M O'Driscoll
Jul 6, 2014·Oncology Reports·Hong-Gang XiangLei Chen
Jan 23, 2013·Journal of Neuro-oncology·Yongli BoWeicheng Yao
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Jan 10, 2013·International Journal of Immunopathology and Pharmacology·S-J PanB-M Sun
Mar 27, 2013·International Journal of Immunopathology and Pharmacology·Y ZhangL-S Jia

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