Expression of P2Y2 purinoceptors in MCG 101 murine sarcoma cells, and HT-29 human colon carcinoma cells

Autonomic Neuroscience : Basic & Clinical
G NylundD S Delbro

Abstract

We investigated how agonists at purinoceptors may affect tumour cell metabolism. This was investigated in vitro in tumour cell lines by microphysiometry, which method monitors extracellular acidification rate (ECAR), on-line. The cell lines investigated were the murine sarcoma, MCG 101, and the human colon cancer, HT-29. In MCG 101, adenosine-5'-triphosphate (ATP) or uridine-5'-triphosphate (UTP) caused a concentration-dependent increase in ECAR, most likely due to the ligation of P2Y(2) receptors, which response was blocked by suramin. In HT-29, ATP or UTP elicited a concentration-dependent, biphasic change in ECAR (increase/decrease). The pharmacological analysis suggests the involvement of P2Y(2) receptors, although other P2 receptor subtypes cannot be entirely excluded. This biphasic response to UTP or ATP was resistant to suramin. The expression of P2Y(2) receptors was demonstrated in both cell lines by immunocytochemistry and Western blot. The current study, thus, shows the functional and morphological expression of a purinoceptor subtype with partly different effects on metabolism in two different tumour cell lines.

References

Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·C E ParrJ T Turner
Dec 19, 1996·European Journal of Pharmacology·D CommuniS Pirotton
Oct 29, 1998·Biochemical and Biophysical Research Communications·M HöpfnerH Scherübl
Nov 14, 1998·Biochimica Et Biophysica Acta·S F PedersenE K Hoffmann
Dec 9, 2000·Naunyn-Schmiedeberg's Archives of Pharmacology·H Zimmermann
Jan 5, 2002·Journal of Pharmacological and Toxicological Methods·S KobayashiK Honda
May 16, 2002·Analytical Biochemistry·Carmen Wiley, Craig Beeson
Sep 6, 2002·Journal of Medicinal Chemistry·Kenneth A JacobsonMichael Williams
Apr 15, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Rainer SchaferGeorg Reiser

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Citations

Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Jun 26, 2008·Pathology Oncology Research : POR·Tamás Deli, László Csernoch
Sep 24, 2005·Autonomic & Autacoid Pharmacology·D S DelbroS Nordgren
Mar 30, 2007·Autonomic & Autacoid Pharmacology·G NylundD S Delbro
May 25, 2005·Cancer Letters·Nicholas WhiteGeoffrey Burnstock
Mar 2, 2006·Pharmacological Reviews·Geoffrey Burnstock
Sep 4, 2019·Purinergic Signalling·Steve Dagenais BellefeuilleFernand-Pierre Gendron

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