Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials.

Scientific Reports
Noelia Ayelen BoccardoFernando Félix Bravo-Almonacid

Abstract

Plants are continuously challenged by pathogens, affecting most staple crops compromising food security. They have evolved different mechanisms to counterattack pathogen infection, including the accumulation of pathogenesis-related (PR) proteins. These proteins have been implicated in active defense, and their overexpression has led to enhanced resistance in nuclear transgenic plants, although in many cases constitutive expression resulted in lesion-mimic phenotypes. We decided to evaluate plastid transformation as an alternative to overcome limitations observed for nuclear transgenic technologies. The advantages include the possibilities to express polycistronic RNAs, to obtain higher protein expression levels, and the impeded gene flow due to the maternal inheritance of the plastome. We transformed Nicotiana tabacum plastids to co-express the tobacco PR proteins AP24 and β-1,3-glucanase. Transplastomic tobacco lines were characterized and subsequently challenged with Rhizoctonia solani, Peronospora hyoscyami f.sp. tabacina and Phytophthora nicotianae. Results showed that transplastomic plants expressing AP24 and β-1,3-glucanase are resistant to R. solani in greenhouse conditions and, furthermore, they are protected against P....Continue Reading

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Citations

Jun 20, 2020·Annual Review of Cell and Developmental Biology·Elia Lacchini, Alain Goossens
Sep 22, 2020·Plant Biotechnology Journal·Micheline N NgakiMadan K Bhattacharyya
Jan 7, 2020·Plant Physiology and Biochemistry : PPB·Prabhjot SinglaSatvir K Grewal
Oct 24, 2020·International Journal of Biological Macromolecules·Yang ZhouDaolong Dou
Apr 4, 2021·The New Phytologist·Chan Man HaRichard A Dixon

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Datasets Mentioned

BETA
KC285905.1
DQ059571.1
DQ067899.1

Methods Mentioned

BETA
transgenic
PCR
electrophoresis
reverse transcription PCR

Software Mentioned

Primer
Gene
GraphPad Prism

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