Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism

Journal of Medical Microbiology
Raúl Manzano-RomanJosé de la Fuente

Abstract

The obligate intracellular pathogen Anaplasma phagocytophilum is transmitted by ticks and causes human granulocytic anaplasmosis, tick-borne fever of ruminants, and equine and canine granulocytic anaplasmosis. In a previous study, the perilipin (PLIN) gene was identified as one of the genes differentially expressed in human promyelocytic HL-60 cells in response to infection with A. phagocytophilum. PLIN is a major adipocyte lipid droplet-associated phosphoprotein that plays a central role in lipolysis and cholesterol synthesis. Host cholesterol and other lipids are required by A. phagocytophilum for infection and multiplication in human cells. In this study, it was hypothesized that PLIN may be involved in infection of human HL-60 cells by A. phagocytophilum. To test this hypothesis, a combination of real-time RT-PCR, immunofluorescence and RNA interference was used to study the expression of PLIN. The results of these studies demonstrated that A. phagocytophilum modulates lipid metabolism by increasing PLIN mRNA levels and facilitates infection of HL-60 cells. The results of these studies expand our knowledge of the role of lipid metabolism in A. phagocytophilum infection and multiplication in HL-60 cells and suggest a mechani...Continue Reading

References

Oct 9, 2003·Cellular Microbiology·Jason A Carlyon, Erol Fikrig
Jan 17, 2004·The Biochemical Journal·Stephen J Yeaman
Mar 8, 2005·Journal of Clinical Microbiology·José de la FuenteKatherine M Kocan
Oct 26, 2005·The Journal of Biological Chemistry·Hsiao-Ping H MooreJames G Granneman
Nov 22, 2005·Infection and Immunity·Bindu SukumaranErol Fikrig
Feb 21, 2006·Emerging Infectious Diseases·J Stephen DumlerJohan S Bakken
Dec 26, 2006·The Journal of Biological Chemistry·James G GrannemanZhengxian Zhu

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Citations

Jun 19, 2012·Future Microbiology·Maiara S SeveroJoao Hf Pedra
Aug 19, 2010·Cellular Microbiology·Bindu SukumaranErol Fikrig
May 23, 2017·Frontiers in Cellular and Infection Microbiology·Dhritiman SamantaStacey D Gilk
Aug 1, 2020·Pathogens·Paige E Allen, Juan J Martinez
Feb 4, 2019·Trends in Parasitology·Alejandro Cabezas-CruzJosé de la Fuente

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