Expression of peroxiredoxins in bovine oocytes and embryos produced in vitro

Molecular Reproduction and Development
Gregory LeyensIsabelle Donnay

Abstract

Peroxiredoxins (PRDXs) form a family of peroxidases involved in antioxidant protection and cell signaling. Due to their peroxide reductase activity, these enzymes might be involved in fine-tuning peroxide levels in embryos during in vitro production. In this study, RT-PCR was used to examine the expression of the six PRDX isoforms (PRDX1 to PRDX6) in bovine oocytes and embryos. PRDXs were detected in oocytes both before and after in vitro maturation. Besides, PRDX6 was up-regulated after maturation. Single embryos were analyzed from the two-cell to the blastocyst stages. PRDX1 and PRDX5 transcripts were detected throughout development. PRDX2, PRDX3, and PRDX6 were not expressed around the 9- to 16-cell stage. PRDX4 transcripts were weakly detected in pools of embryos from the 9- to 16-cell stage onwards. In situ immunodetection of PRDX5, which was previously reported to exhibit the widest subcellular distribution among PRDXs in adult mammalian cells, showed a mitochondrial distribution pattern in the bovine embryo. Finally, the potential modulation by oxidative stress of PRDX expression around the major embryonic genome activation was evaluated by culturing embryos under 20% O2 instead of 5%. No significant difference in the pa...Continue Reading

References

Apr 1, 1991·Differentiation; Research in Biological Diversity·G B PierceA L Lewellyn
Nov 1, 1993·Trends in Biochemical Sciences·W R Engels
Mar 1, 1996·Journal of Reproduction and Fertility·J G ThompsonH J Leese
Jan 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D K BarnettB D Bavister
Jan 1, 1997·Reviews of Reproduction·E de LamirandeC Gagnon
Sep 25, 1998·The Journal of Biological Chemistry·A K Singh, H Shichi
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S AkibaA B Fisher
May 20, 1999·Molecular Reproduction and Development·I Donnay, H J Leese
Jun 25, 1999·Cell Death and Differentiation·S Pampfer, I Donnay
Jul 20, 1999·The Journal of Biological Chemistry·A B FisherS I Feinstein
Jan 6, 2001·Molecular Reproduction and Development·A S LequarréA Van Langendonckt
Feb 22, 2001·Free Radical Biology & Medicine·T D OberleyS G Rhee
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·H S KimL B Clerch
Jun 9, 2001·Biochemical and Biophysical Research Communications·M X WangG A Murrell
Jun 27, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·Q Y SunH Schatten
Aug 18, 2001·American Journal of Respiratory Cell and Molecular Biology·K C DasC W White
Jan 25, 2002·IUBMB Life·S G RheeK Kim
Mar 27, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·Alexandra J HarveyJeremy G Thompson
Oct 23, 2002·Biology of Reproduction·Claude RobertMarc-André Sirard
Jan 9, 2003·Trends in Biochemical Sciences·Zachary A WoodLeslie B Poole
Mar 11, 2003·Zygote : the Biology of Gametes and Early Embryos·J KankaJ P Renard
Apr 26, 2003·Science·Zachary A WoodP Andrew Karplus
Jul 10, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Han-Suk KimAron B Fisher
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Sue Goo RheeSang Won Kang
Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Gregory LeyensBernard Knoops
Dec 24, 2003·Investigative Ophthalmology & Visual Science·Ying PengMarie C Lin

❮ Previous
Next ❯

Citations

Sep 1, 2005·Apoptosis : an International Journal on Programmed Cell Death·S K ChaubeT G Shrivastav
Aug 20, 2010·Journal of Assisted Reproduction and Genetics·Maw-Sheng LeeEn-Hui Cheng
Jan 26, 2012·Journal of Assisted Reproduction and Genetics·Catherine M H CombellesCatherine Racowsky
Oct 28, 2010·Antioxidants & Redox Signaling·Bernard KnoopsJean-Paul Declercq
Mar 31, 2006·Human Reproduction·Said AssouSamir Hamamah
Jan 19, 2012·PloS One·Henriette PoatyAlain Bernheim
Sep 12, 2013·PloS One·Valérie Van der EeckenBernard Knoops
Apr 24, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Takako YazakiEimei Sato
Sep 17, 2011·Gene Expression Patterns : GEP·Maxwell E R ShaferSashko Damjanovski
Jan 22, 2008·Free Radical Biology & Medicine·Michael R GarrySamir S Deeb
Oct 27, 2007·Biochimica Et Biophysica Acta·Yan MengJia-Hao Sha
Mar 1, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Tamar ZivArie Admon
Nov 10, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Shie WangJianxin Wang
Aug 22, 2014·Proteomics·Myriam DemantGeorg J Arnold
Jan 17, 2015·Animal Reproduction Science·S A ApichelaC Stelletta
Jun 11, 2014·Zygote : the Biology of Gametes and Early Embryos·Gustavo Bruno MotaAlzira Amélia Martins Rosa E Silva
Aug 9, 2006·Molecular Reproduction and Development·Marina MourotMarc-André Sirard
Sep 13, 2013·Reproduction, Fertility, and Development·Mateus J SudanoFernanda D C Landim-Alvarenga
Jan 30, 2015·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D K de SouzaA A M Rosa e Silva
Feb 23, 2020·International Journal of Molecular Sciences·Stefan BauersachsCarmen Almiñana
Feb 5, 2019·Human Reproduction·Mandy SpaanUNKNOWN OMEGA-steering group
Apr 25, 2015·The Veterinary Record·M L H Campbell, P Sandøe
Apr 21, 2010·Reproduction in Domestic Animals = Zuchthygiene·R RomarR Dalbiès-Tran
Sep 26, 2020·Scientific Reports·Camila Bruna de LimaMarcella Pecora Milazzotto
Jan 6, 2018·Nature Communications·Jean-Louis GuéantDavid S Rosenblatt
Jan 9, 2021·Research in Veterinary Science·Yentel Mateo-OteroElisa Giaretta
Jan 22, 2009·Journal of Proteome Research·Jae-Kyung MyungGert Lubec
Jun 6, 2009·Reproductive Biomedicine Online·Catherine M H CombellesAshok Agarwal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.