Expression of phospholipase A2 group IVA (PLA2G4A) is upregulated by human chorionic gonadotropin in bovine granulosa cells of ovulatory follicles

Biology of Reproduction
Mame Nahé DioufJacques G Lussier

Abstract

Prostaglandins are required for the ovulatory process, and their biosynthesis depends on the initial release of arachidonic acid from membrane phospholipids. We hypothesized that phospholipase A2 group IVA (PLA2G4A) expression is upregulated in granulosa cells (GC) at ovulation. We have characterized bovine PLA2G4A cDNA, and investigated its spatiotemporal regulation at the mRNA and protein levels in hCG-induced ovulatory follicles and in vitro, using forskolin-stimulated GC. Regulation of PLA2G4A mRNA expression was studied in GC obtained from bovine follicles collected at different developmental stages: small follicles (2-4 mm), dominant follicles at Day 5 (D5) of the estrous cycle, ovulatory follicles 24 h following injection of hCG, and corpus luteum at D5. PLA2G4A mRNA increased by 14-fold in GC of hCG-stimulated versus dominant follicles (P < 0.0001). Follicular walls obtained from ovulatory follicles recovered at 0, 6, 12, 18, and 24 h post-hCG injection showed an initial 16-fold increase in PLA2G4A transcript at 12 h that reached a 45-fold increase at 24 h, as compared to 0 h (P < 0.0001). Immunoblots of GC extracts showed an initial induction of the PLA2G4A protein at 18 h post-hCG, reaching a maximum at 24 h. Immunohi...Continue Reading

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Citations

Jun 8, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shiro KurusuJoseph V Bonventre
Feb 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Khampoune SayasithJean Sirois
Feb 28, 2013·Zygote : the Biology of Gametes and Early Embryos·Maria Eugenia OrtizLiliana Isabel Zelarayán
Aug 26, 2009·Prostaglandins & Other Lipid Mediators·Shiro KurusuMitsumori Kawaminami
Feb 6, 2014·Animal Genetics·L R Porto-NetoC Gondro
May 31, 2015·Human Reproduction Update·Diane M Duffy
Jun 25, 2009·Molecular Reproduction and Development·Andrea L Lister, Glen J Van Der Kraak
Sep 6, 2013·Biology of Reproduction·Khampoun SayasithJacques G Lussier
Mar 11, 2020·Zygote : the Biology of Gametes and Early Embryos·Ahmed S A SosaMahmoud F Nawito
Nov 5, 2017·Reproductive Biology and Endocrinology : RB&E·Jacques G LussierKalidou Ndiaye

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AAF15299

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PCR
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