Expression of phospholipases A2 in primary human lung macrophages: role of cytosolic phospholipase A2-alpha in arachidonic acid release and platelet activating factor synthesis.

Biochimica Et Biophysica Acta
Giorgio GiannattasioMassimo Triggiani

Abstract

Macrophages are a major source of lipid mediators in the human lung. Expression and contribution of cytosolic (cPLA(2)) and secreted phospholipases A(2) (sPLA(2)) to the generation of lipid mediators in human macrophages are unclear. We investigated the expression and role of different PLA(2)s in the production of lipid mediators in primary human lung macrophages. Macrophages express the alpha, but not the zeta isoform of group IV and group VIA cPLA(2) (iPLA(2)). Two structurally-divergent inhibitors of group IV cPLA(2) completely block arachidonic acid release by macrophages in response to non-physiological (Ca(2+) ionophores and phorbol esters) and physiological agonists (lipopolysaccharide and Mycobacterium protein derivative). These inhibitors also reduce by 70% the synthesis of platelet-activating factor by activated macrophages. Among the full set of human sPLA(2)s, macrophages express group IIA, IID, IIE, IIF, V, X and XIIA, but not group IB and III enzymes. Me-Indoxam, a potent and cell impermeable inhibitor of several sPLA(2)s, has no effect on arachidonate release or platelet-activating factor production. Agonist-induced exocytosis is not influenced by cPLA(2) inhibitors at concentrations that block arachidonic acid r...Continue Reading

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Citations

Oct 4, 2013·Mediators of Inflammation·Ewa Pniewska, Rafal Pawliczak
Jun 11, 2016·Scientific Reports·Preeti BansalNaveen Arora
Jul 7, 2009·Biochimica Et Biophysica Acta·Eirini KitsiouliMarilena E Lekka
Apr 1, 2015·Infection and Immunity·Carole GuillaumeChristiane Deregnaucourt
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Mar 6, 2016·Journal of Nanobiotechnology·Martin WiemannRobert Landsiedel
Dec 25, 2013·The Journal of Biological Chemistry·Milena SokolowskaJames H Shelhamer
Jan 3, 2020·Cellular and Molecular Life Sciences : CMLS·Markus WernerOliver Werz

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