Expression of plasma membrane Ca2+ ATPase family members and associated synaptic proteins in acute and cultured organotypic hippocampal slices from rat

Brain Research. Developmental Brain Research
Thomas P JensenRuth M Empson

Abstract

Plasma membrane Ca2+ ATPases (PMCAs) are critical regulators of intracellular Ca2+ concentration ([Ca2+]i). Specific isoforms have also been demonstrated to interact and co-localise with members of the synapse-associated protein (SAP) family in hippocampal dendritic spines. Presently, only indirect evidence of changes in PMCA protein expression during postnatal development exists, therefore we chose to examine the postnatal developmental protein expression patterns of PMCAs 1-4 and the SAP proteins SAP102 and PSD95. Using Western blotting analysis, we compared the postnatal expression in the in vivo hippocampus to the expression within in vitro organotypic hippocampal slice cultures; a valid model of the developing hippocampus. All PMCA and SAP family members studied showed a marked increase in protein expression levels throughout the postnatal time course both in vivo and in vitro. SAP102 and the ubiquitously expressed PMCAs 1 and 4 followed a similar time course of expression within the in vivo and in vitro preparations. In contrast, the neurone-specific PMCA isoforms 2 and 3 and PSD95 displayed slight differences in early postnatal development. However, and most importantly, their expression > or = 14 days in vitro (DIV) was...Continue Reading

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Citations

Nov 30, 2013·International Journal of Molecular Sciences·Eui-Ju Hong, Eui-Bae Jeung
May 10, 2012·Experimental Neurology·Shigeki Tsutsui, Peter K Stys
Feb 18, 2012·Cold Spring Harbor Perspectives in Biology·Michael J Higley, Bernardo L Sabatini
Jul 2, 2008·The Journal of Physiology·Gabriella StoccaJosef Bischofberger
Sep 23, 2014·The Journal of Physiology·Jörg Pohle, Josef Bischofberger
May 5, 2009·Molecular and Cellular Neurosciences·Thomas P JensenRuth M Empson
Jun 26, 2009·Progress in Neurobiology·Laura LossiAdalberto Merighi
Apr 2, 2015·IET Nanobiotechnology·Samaneh DavoudiMahdi Zaeifi Yamchi
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Nov 4, 2020·Insects·Priyadarshini Chakrabarti, Ramesh R Sagili
Oct 19, 2010·European Journal of Medicinal Chemistry·Chinmoy MukherjeeAnup Kumar Misra

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