Expression of pluripotent stem cell markers in the human fetal ovary

Human Reproduction
Candace L KerrJohn D Gearhart

Abstract

Human primordial germ cells (PGCs) can give rise to pluripotent stem cells such as embryonal carcinoma cells (ECCs) and embryonic germ cells (EGCs). In order to determine whether PGCs express markers associated with pluripotency in EGCs and ECCs, the following study cross examines the expression patterns of multiple pluripotent markers in the human fetal ovary, 5.5-15 weeks post-fertilizaton (pF) and relates this expression with the ability to derive pluripotent EGCs in vitro. Specific subpopulations were identified which included OCT4(+)/Nanog(+)/cKIT(+)/VASA(+) PGCs and oogonia. Interestingly, these cells also expressed SSEA1 and alkaline phosphatase (AP) and SSEA4 expression occurred throughout the entire gonad. Isolation of SSEA1(+) cells from the gonad resulted in AP(+) EGC colony formation. The number of OCT4(+) or Nanog(+) expressing cells peaked by week 8 and then diminished after week 9 pF, as oogonia enter meiosis. In addition, the efficiency of EGC derivation was associated with the number of OCT4(+) cells. TRA-1-60 and TRA-1-81 were only detected in the lining of the mesonephric ducts and occasionally in the gonad. These results demonstrate that PGCs, a unipotent cell, express most, but not all, of the markers assoc...Continue Reading

Citations

May 22, 2010·Stem Cells and Development·Marc HillerCandace L Kerr
Jul 31, 2010·Human Reproduction·R Le BouffantG Livera
Aug 28, 2009·Molecular Human Reproduction·Candace TingenTeresa K Woodruff
Apr 29, 2009·PloS One·Kaori YamauchiHirofumi Suemori
Sep 20, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·F J StansfieldW R Allen
Feb 4, 2014·Differentiation; Research in Biological Diversity·Yang LiJichun Tan
May 21, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·B FereydouniR Behr
Jul 30, 2010·Stem Cell Reviews and Reports·Maria P De MiguelYago Alcaina
Jun 5, 2012·Fertility and Sterility·Yohei HayashiShinya Yamanaka
Sep 28, 2015·Seminars in Cell & Developmental Biology·Anne JørgensenEwa Rajpert-De Meyts
Oct 13, 2015·Seminars in Cell & Developmental Biology·Susanna DolciMassimo De Felici
Nov 4, 2015·Frontiers in Genetics·Emanuele PelosiDavid Schlessinger
Mar 24, 2009·Birth Defects Research. Part C, Embryo Today : Reviews·Martine Culty
Sep 20, 2008·Stem Cells·Katarzyna TilgnerLyle Armstrong
May 3, 2008·Differentiation; Research in Biological Diversity·Irma Virant-KlunHelena Meden-Vrtovec
Aug 6, 2014·Anatomical Science International·Mohammad Ghasemzadeh-HasankolaeiMaryam Ghasemzadeh-Hasankolaei
Jan 14, 2012·Molecular and Cellular Endocrinology·Ranna El-KhairiJohn C Achermann
Jan 31, 2015·Molecular Human Reproduction·Galbha DuggalPetra De Sutter
Aug 10, 2016·In Vitro Cellular & Developmental Biology. Animal·Zohreh AfsartalaMohammad Ghasemzadeh-Hasankolaei
Aug 31, 2016·Nature Reviews. Genetics·Walfred W C TangM Azim Surani
Oct 8, 2016·Clinical Genetics·Remko HersmusLeendert H J Looijenga
Sep 12, 2015·Biology of Reproduction·Darryl L Russell, Raymond J Rodgers
Jun 10, 2011·Biology of Reproduction·Danielle E HickfordMarilyn B Renfree
Dec 30, 2014·Endocrine Reviews·Katja HummitzschRaymond J Rodgers
Dec 28, 2018·Endocrine Reviews·Juho-Antti MäkeläJorma Toppari
Oct 27, 2011·Médecine sciences : M/S·Maëlle Pannetier, Eric Pailhoux
Jun 22, 2019·Biochemistry. Biokhimii︠a︡·V K AbdyyevA V Vasiliev
Aug 11, 2020·ELife·Bogdan Andrei MirautaAngus I Lamond
Mar 23, 2019·Genes & Cancer·Candace L KerrVenu Raman
Nov 6, 2014·Biomolecular Concepts·Anne Jørgensen, Ewa Rajpert-De Meyts
Feb 16, 2017·Cellular and Molecular Life Sciences : CMLS·Chao WangGuoliang Xia
Mar 1, 2009·Expert Review of Endocrinology & Metabolism·Zeev Blumenfeld

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