Expression of poplar chitinase in tomato leads to inhibition of development in colorado potato beetle

Biotechnology Letters
Susan D Lawrence, Nicole Novak

Abstract

The previously described poplar chitinase, WIN6, is induced during infestation by gypsy moth (Lymantria dispar L.) larvae, thus suggesting a role in defense against insect pests. To test this hypothesis, we produced tomato seedlings infected with a recombinant potato virus X (PVX), which produces WIN6, and tested its insecticidal properties on Colorado potato beetle [CPB; Leptinotarsa decemlineata (Say)], which is a serious pest of tomatoes and other crops. The advantage of PVX is that plant material is ready for insect bioassay within 3-4 weeks of constructing the recombinant virus. Considering that production of transgenic tomato seedlings using Agrobacterium takes at least 6 months, this hastens the rate at which genes can be examined. Upon insect bioassay, only 47% CPB neonates feeding on leaves containing >0.3% w/w WIN6 developed to 2nd instar while 93% of controls reached 2nd instar. To our knowledge this is the first plant chitinase that retards development of an insect pest.

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Citations

Sep 7, 2006·Biotechnology Letters·Susan D LawrenceJohn M Davis
Mar 5, 2008·Plant Physiology·Keyan Zhu-SalzmanGary W Felton
Mar 14, 2014·PloS One·Kotaro KonnoMasatoshi Nakamura
Sep 29, 2011·Yi chuan = Hereditas·Ao-Xue Wang, Xiu-Ling Chen
Oct 28, 2015·International Journal of Molecular Sciences·Weiping FangXiaojie Yang
Jan 1, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Lei Wang, Jianqiang Wu
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May 14, 2016·Planta·Beata Wielkopolan, Aleksandra Obrępalska-Stęplowska
May 18, 2013·International Journal of Molecular Sciences·Joel Fürstenberg-HäggSøren Bak
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