Expression of prepro-GnRH and GnRH receptor messengers in rainbow trout ovary depends on the stage of ovarian follicular development

Molecular Reproduction and Development
S UzbekovaB Breton

Abstract

Gonadotropin-Releasing Hormones (GnRHs) are decapeptides well known to regulate the reproductive cycle. They are expressed not only in the brain, but also in other tissues including the gonads. It is believed that they may be involved in the endocrine and paracrine regulation of the reproductive cycle. To date, two forms of GnRH have been identified in salmonids: salmon (sGnRH) and chicken II (cGnRH-II). In the present study, the temporal expression of sGnRH-1, sGnRH-2, cGnRH-II, and rtGnRH receptor genes was studied in rainbow trout ovary during the reproductive cycle according to the stages of follicular development. Using RT-PCR coupled with Southern-blot hybridization, sGnRH-1, sGnRH-2, cGnRH-II, and rtGnRH-R transcripts were detected in morphologically nondifferentiated ovaries as early as 55-65 days post-fertilization and throughout all stages of vitellogenesis. Using Northern blot analysis, cGnRH-II mRNA was detected only in immature previtellogenic ovary, whereas sGnRH mRNA was detected also during early and mid-exogenous vitellogenesis. No sGnRH mRNA was detected at the end of vitellogenesis. In maturing pre-ovulated ovary, sGnRH transiently reappeared before germinal vesicle breakdown (GVBD) and decreased thereafter. ...Continue Reading

References

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May 4, 2001·Molecular Reproduction and Development·S UzbekovaJ J Lareyre
Jun 12, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S UzbekovaB Breton
Jun 12, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C V Andreu-Vieyra, H R Habibi

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Citations

Jul 10, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Padmasana SinghRajagopala Sridaran
Sep 3, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Huamei YueChuangju Li
Oct 6, 2012·General and Comparative Endocrinology·H MigaudA Davie
Apr 10, 2012·General and Comparative Endocrinology·David A Irwin, Glen Van Der Kraak
Sep 24, 2008·General and Comparative Endocrinology·Padmasana SinghKazuyoshi Tsutsui
Jun 8, 2012·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Yong-Jiang XuQing-Yin Wang
May 24, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Leonardo G GuilgurGustavo M Somoza
Jul 30, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ying SongLuqing Pan
Nov 1, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Andrea MiccoliOliana Carnevali

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