Abstract
Sperm-associated antigen 6 (Spag6) gene, which encodes an axonemal protein (SPAG6), ubiquitously expresses in tissue and organs containing ciliated cells. The present work was to investigate whether SPAG6 expressed in cochlear hair cells and, if so, to explore the presumable correlations between prestin and SPAG6. The distribution of SPAG6 in organ of Corti and the morphological features of hair cells in basilar membrane were investigated by immunofluorescent staining. The amount of prestin in Spag6 mutant mice was measured by Western blotting and real-time PCR, respectively. Additionally, co-immunoprecipitation tests were performed to confirm the presumed interaction between prestin and SPAG6. We observed that SPAG6 expressed in the cuticular plate in outer hair cells (OHCs) and prestin in the lateral wall of OHCs that located along with SPAG6 at this site. In comparison to Spag6 +/+ mice, Spag6 -/- mice showed apparent morphological abnormity of OHCs and lower intensity of prestin fluorescence. The expression of prestin in Spag6 -/- mice reduced significantly at both protein and mRNA levels. Moreover, co-immunoprecipitation tests demonstrated the interaction between prestin and SPAG6. Taken together, these data indicate that ...Continue Reading
References
Jun 1, 1994·Hearing Research·Y RaphaelR A Altschuler
Sep 24, 1999·Genomics·L I NeilsonJ F Strauss
Feb 1, 2000·European Journal of Pediatrics·D HolzmannH Felix
May 23, 2000·Nature·J ZhengP Dallos
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 9, 2002·Molecular and Cellular Biology·Rossana SapiroJerome F Strauss III
Sep 20, 2002·Nature·M Charles LibermanJian Zuo
Oct 23, 2002·Molecular and Cellular Biology·Zhibing ZhangJerome F Strauss
Jan 15, 2005·The Journal of Physiology·Levente DeákPeter Dallos
Aug 23, 2005·Biophysical Journal·Dhasakumar NavaratnamJoseph Santos-Sacchi
May 20, 2006·Brain Research·Ning YuHong-Bo Zhao
Feb 27, 2007·Cell Motility and the Cytoskeleton·Zhibing ZhangJerome F Strauss
Apr 20, 2007·Developmental Neurobiology·Xudong WuJian Zuo
Apr 27, 2010·The Journal of Biological Chemistry·Jun-Ping BaiDhasakumar Navaratnam
Jul 14, 2010·The Journal of Cell Biology·Martin SchwanderUlrich Müller
Feb 24, 2011·Developmental Neurobiology·Johann GrossBirgit Mazurek
Apr 27, 2011·Anatomy & Cell Biology·Heejei YoonJinwoong Bok
May 27, 2011·Cellular and Molecular Neurobiology·Johann GrossBirgit Mazurek
Citations
Feb 3, 2016·Neuroscience Letters·Qianqian YangJianfeng Li
Jan 20, 2016·Cytoskeleton·Maria E TevesJerome F Strauss
Sep 27, 2018·Journal of Cellular and Molecular Medicine·Meijuan ZhouHaibo Wang
Nov 12, 2020·American Journal of Physiology. Cell Physiology·Xiaofei LiHaibo Wang
Oct 30, 2019·Molecular Therapy Oncolytics·Da-Fang ZhengYi-Sheng Tao
Nov 5, 2020·Poultry Science·Z R JarrellA P Benson