Expression of profibrotic mediators in small airways versus parenchyma after cigarette smoke exposure

American Journal of Respiratory Cell and Molecular Biology
Andrew ChurgJoanne L Wright

Abstract

Cigarette smoke-induced lung disease presents a morphologic contradiction in that the small airways become fibrotic but the parenchyma becomes emphysematous over time. To examine the mechanisms behind these phenomena, we exposed mice to cigarette smoke for up to 6 months and isolated small airways from histologic sections by laser capture microdissection. We then removed residual airway tissue and vessels, and collected the remaining parenchymal tissue. Gene expression of 13 fibrogenic growth/signaling factors (particularly TGF-beta-related genes), matrix proteins, or enzymes involved in matrix production was examined by real-time RT-PCR. Combining present and previously published data from our laboratory, in the airways over the long term there was a sustained and marked increase in expression of almost all of these genes. By contrast, in the parenchyma, expression of most genes was elevated at 2 and 24 hours after initial exposure, and all were elevated at 1 month; but by 6 months, when emphysema was present, most genes (9/13) were either at control values or down-regulated below control. At 3 months, several genes that were considerably elevated at 1 month were back to control levels, suggesting that loss of the parenchymal ...Continue Reading

References

Dec 1, 1990·The American Review of Respiratory Disease·J L Wright, A Churg
Jan 1, 1989·Journal of Clinical Pathology·A Kennedy, A K Foulis
Mar 22, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·O EickelbergM Roth
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·P K Jeffery
Apr 23, 2003·American Journal of Respiratory Cell and Molecular Biology·Rubin M TuderPeter M Henson
Feb 11, 2004·Chest·Naoko YokohoriUNKNOWN Respiratory Failure Research Group in Japan
May 1, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew Leask, David J Abraham
Jun 9, 2004·American Journal of Respiratory and Critical Care Medicine·Andrew ChurgJoanne L Wright
Jun 23, 2004·Cytokine & Growth Factor Reviews·James C Bonner
Jul 10, 2004·American Journal of Respiratory Cell and Molecular Biology·Joanne L WrightAndrew Churg
Jul 31, 2004·American Journal of Respiratory Cell and Molecular Biology·Heiko A GolponNorbert F Voelkel
Sep 18, 2004·American Journal of Respiratory Cell and Molecular Biology·Avrum SpiraJerome S Brody
Oct 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wen NingAugustine M K Choi
Feb 3, 2005·The European Respiratory Journal·K ImaiJ M D'Armiento
Apr 20, 2005·American Journal of Respiratory Cell and Molecular Biology·Steven D Shapiro, Edward P Ingenito
Jul 16, 2005·Experimental Lung Research·Robert F ForonjyYasunori Okada
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Xin-Hua Feng, Rik Derynck
Nov 15, 2005·The Journal of Clinical Investigation·Bing MaJack A Elias
Jun 27, 2006·Proceedings of the American Thoracic Society·Dirkje S Postma, Wim Timens
Aug 16, 2006·Cellular and Molecular Life Sciences : CMLS·H A Lucero, H M Kagan
Sep 30, 2006·American Journal of Respiratory and Critical Care Medicine·Andrew ChurgJoanne L Wright
Oct 27, 2006·Proceedings of the American Thoracic Society·Stephen I RennardOlaf Holz
Dec 5, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Adrian ShifrenRobert P Mecham
Jan 9, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ping-Ping KuangRonald H Goldstein
Jan 24, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Min-Jong KangJack A Elias
Feb 20, 2007·Current Drug Targets. Inflammation and Allergy·Teresa D Tetley
Aug 28, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christopher S StevensonKeith Butler
Nov 3, 2007·American Journal of Respiratory and Critical Care Medicine·I-Ming WangPeter D Paré
Jan 29, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andrew ChurgJoanne L Wright
May 6, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Joanne L WrightAndrew Churg

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Citations

Oct 3, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Yu-long LuoWei-wei Su
Feb 3, 2011·Lung·Lina María Salazar, Ana Milena Herrera
Dec 17, 2009·American Journal of Respiratory and Critical Care Medicine·Ali O YildirimHeinz Fehrenbach
Jan 16, 2010·American Journal of Respiratory and Critical Care Medicine·John V GosselinkJames C Hogg
Aug 17, 2010·American Journal of Respiratory and Critical Care Medicine·Joanne L WrightAndrew M Churg
Oct 22, 2008·American Journal of Respiratory Cell and Molecular Biology·Andrew ChurgJoanne L Wright
Dec 2, 2010·Respiratory Research·Terttu HarjuRiitta Kaarteenaho
May 15, 2013·PloS One·Lisette I Z KunzUNKNOWN GLUCOLD (Groningen Leiden Universities Corticosteroids in Obstructive Lung Disease) Study Group
Sep 9, 2010·Chest·Richard J CastriottaKevin P High
Jul 1, 2015·Archivum Immunologiae Et Therapiae Experimentalis·Katarzyna GrzelaTomasz Grzela
Aug 10, 2011·Pulmonary Pharmacology & Therapeutics·Robin J McAnulty
Jul 12, 2011·Revue des maladies respiratoires·P-R BurgelI Tillie-Leblond
Oct 6, 2009·Free Radical Biology & Medicine·R-M Liu, K A Gaston Pravia
Apr 24, 2015·Lung·Joseph LeungNarasaiah Kolliputi
Mar 31, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Shi LiJinnong Zhang
Dec 30, 2014·The American Journal of Pathology·Francesca PolverinoCaroline A Owen
Nov 4, 2009·Inhalation Toxicology·Yolanda S López-BoadoAndrew Churg
Jun 24, 2017·Pharmacology & Therapeutics·Rama MalaviyaDebra L Laskin
Jun 25, 2010·Advances in Anatomic Pathology·Mary Beth Beasley
Feb 12, 2019·American Journal of Respiratory and Critical Care Medicine·Brian J SandriOla Larsson
Feb 1, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Simon D PouwelsMartijn C Nawijn
Jun 9, 2017·Journal of Breath Research·Todor A PopovDavor Plavec
Jul 12, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Laszlo FarkasMartin Kolb
Jul 12, 2017·Archives of Physiology and Biochemistry·Tanya TachevaTatyana Vlaykova
Jun 6, 2013·Expert Review of Respiratory Medicine·Anthony Tam, Don D Sin
Dec 7, 2010·Expert Review of Respiratory Medicine·Joanne L Wright, Andrew Churg
Dec 23, 2009·Current Opinion in Allergy and Clinical Immunology
Jan 30, 2010·Current Opinion in Pulmonary Medicine

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