Expression of prolactin receptors in human osteosarcoma cells

Biochemical and Biophysical Research Communications
N Bataille-SimoneauL Mercier

Abstract

The expression of prolactin receptor (PRL-R) mRNA was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) combined with Southern analysis in total RNA extracts from two human osteosarcoma cell lines (MG-63 and Saos-2). The level of PRL-R transcript was significantly enhanced in cells cultured in the presence of 1,25-(OH)2 vitaminD3 (10(-7)M) and to a lesser extent in the presence of dexamethasone (10(-6)M). This first demonstration of PRL-R gene expression in osteoblast-like cells supports the hypothesis of a direct action of prolactin in bone cells, which is further strongly suggested by the stimulatory effect of 1,25-(OH)2 vitaminD3 and dexamethasone on PRL-R mRNA level in these cells.

Citations

Sep 13, 2008·Calcified Tissue International·Gregory J TranahUNKNOWN Study of Osteoporotic Fractures (SOF) Research Group
Sep 25, 2012·Current Osteoporosis Reports·Monique J Brown, Briana Mezuk
Apr 25, 2008·Canadian Journal of Physiology and Pharmacology·Narattaphol CharoenphandhuNateetip Krishnamra
Mar 10, 2011·Expert Opinion on Drug Safety·Simon Matthew GrahamEvangelia Tsapakis
Apr 15, 2009·Journal of Cellular Biochemistry·Dutmanee SeriwatanachaiJ P T M van Leeuwen
Apr 24, 2018·Endocrine Reviews·Gherardo MazziottiAndrea Giustina
Sep 28, 2006·American Journal of Physiology. Endocrinology and Metabolism·Narattaphol CharoenphandhuNateetip Krishnamra
Nov 30, 2000·American Journal of Physiology. Endocrinology and Metabolism·D CossA M Walker
Dec 1, 2010·Molecular and Cellular Biochemistry·Kannikar WongdeeNarattaphol Charoenphandhu
Mar 29, 2020·Pituitary·Luigi di FilippoAndrea Giustina
Dec 22, 1999·Genetic Analysis : Biomolecular Engineering·V GoffinP A Kelly
Oct 29, 2020·Seminars in Cell & Developmental Biology·Ödül Karayazi AtıcıCarrie S Shemanko
Apr 27, 2021·Endocrine Reviews·Edouard G MillsAlexander N Comninos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.