PMID: 8940659Aug 1, 1996Paper

Expression of protamine P2 in the testis of the common marmoset and man visualized using non-radioactive in-situ hybridization

International Journal of Andrology
P T SaundersM R Millar

Abstract

Information on the organization of the spermatogenic cycle of the common marmoset (Callíthrix jacchus), a small New World primate, is limited to a single histological report on the differentiation of spermatids. In the present study we have used non-radioactive in-situ hybridization with a cRNA probe directed against marmoset protamine 2, on fixed sections of marmoset and human testis to elucidate the organization of mature germ cells within the seminiferous epithelium. Specificity of the probe was checked on Northern blots; mP2 hybridized exclusively to mRNA in samples extracted from marmoset and human testis. In sections from human and marmoset testis, positive staining for mRNA was confined to round and elongating spermatids and in the human was reduced in samples from patients with incomplete spermatogenesis. In the human, P2 mRNA was present in groups of cells consistent with the presence of more than one stage of the spermatogenic cycle in transverse sections of individual tubules. In the marmoset, P2-positive cells were detected as a continuous ring of staining in the majority of sections of tubules whilst in others only a group(s) of cells was positive. We conclude that the arrangement of the spermatogenic wave in this ...Continue Reading

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Citations

Sep 30, 2000·International Journal of Andrology·M R MillarP T Saunders
Feb 29, 2000·Molecular Human Reproduction·K StegerM Bergmann
Sep 1, 2018·Systems Biology in Reproductive Medicine·Rod BalhornMonique C Balhorn
Sep 13, 2000·Nature·S Lloyd
Jun 12, 2010·Systems Biology in Reproductive Medicine·Laszlo NanassyDouglas T Carrell
Jul 7, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Vivien M KendonWilliam J Munro
Jul 5, 2017·Reports on Progress in Physics·Joonwoo Bae

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