PMID: 7538980May 16, 1995Paper

Expression of protectin (CD59) in human melanoma and its functional role in cell- and complement-mediated cytotoxicity

International Journal of Cancer. Journal International Du Cancer
L I BrasoveanuA Cattelan

Abstract

Immunohistochemical and/or indirect immunofluorescence analysis with monoclonal antibody (MAb) H19 demonstrated the expression of protectin (CD59) in 54 surgically removed metastatic melanoma lesions and on 8 out of 12 melanoma cell lines. CD59 expression had a low degree of intra- and intertumor heterogeneity. SDS-PAGE analysis showed that the molecular weight of CD59 expressed on melanoma cells is about 20 kDa. Treatment of melanoma cells with 5U/ml of phosphatidylinositol-specific phospholipase C completely abolished cell-surface expression of CD59. Interferon-gamma and/or tumor necrosis factor-alpha or phorbol 12-myristate 13-acetate neither modulated the expression of CD59 by melanoma cells nor influenced the amounts of CD59-specific mRNA. F(ab')2 fragments of anti-CD59 MAb YTH53. I did not inhibit the lysis of melanoma cells by allogeneic natural killer (NK) cells or lymphokine-activated killer (LAK) cells. In contrast, the whole Ig molecule of MAb HI9 or YTH53.I significantly (p < 0.05) enhanced NK-cell-mediated lysis of melanoma cells, suggesting the induction of antibody-dependent cell-mediated cytotoxicity. Lastly, masking of CD59 by MAb YTH53.I or its F(ab')2 fragments significantly (p < 0.05) enhanced, in a dose-dep...Continue Reading

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Citations

Aug 24, 2001·International Journal of Cancer. Journal International Du Cancer·K JurianzM Kirschfink
Apr 14, 2006·Cancer Immunology, Immunotherapy : CII·Daniel J HellrungCharles J Link
Mar 4, 2000·Molecular Immunology·K JurianzM Kirschfink
Sep 9, 1998·American Journal of Respiratory Cell and Molecular Biology·S VarsanoJ Radnay
Jul 17, 1999·Journal of Cutaneous Pathology·M WeichenthalE W Breitbart
Jan 20, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Naren M H Ravindranath, Charles Shuler
Sep 19, 2008·International Journal of Cancer. Journal International Du Cancer·Lydie CassardCatherine Sautès-Fridman
Apr 17, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Rajat BannerjiJohn C Byrd
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Dec 24, 1997·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·D J BlomM J Jager

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