Expression of prothrombinase activity and CD9 antigen on the surface of small vesicles from stimulated human endothelial cells

Thrombosis Research
H KagawaH Takahashi

Abstract

We employed flow cytometry and monoclonal antibodies (MoAb) to study the surface membrane protein of shed particles (small vesicles, SV) that were released from vascular endothelial cells (EC) by agonists such as a Ca ionophore (A23187) and thrombin. After stimulation of EC by A23187, CD9 antigens disappeared entirely from the EC surface in a time- and concentration-dependent manner; they subsequently moved onto the SV surface. Von Willebrand factor (vWF) and P-selectin from Weibel-Palade (W-P) bodies were expressed rapidly on the EC surface after thrombin stimulation, but not on the SV surface. P-selectin may have some effect on maintenance of hemostasis on the EC surface. We demonstrated that the surfaces of SV and EC significantly supported prothrombinase activity and confirmed that A23187-induced SV from EC express binding sites for factors IXa and Xa. These results suggest that the SV are an important factor in a novel controlling mechanism of the coagulation system on the EC surface.

References

May 1, 1991·British Journal of Haematology·M YanabuK Yasunaga
Jun 1, 1983·The Journal of Clinical Investigation·M ColucciN Semeraro
Jan 1, 1994·Seminars in Thrombosis and Hemostasis·W F GlassJ I Kreisberg
Feb 1, 1959·The American Journal of Physiology·H S BENNETTJ C HAMPTON

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Citations

Mar 9, 2004·Blood Cells, Molecules & Diseases·Saskia H H F SchoenmakersC Arnold Spek
Nov 1, 2015·Seminars in Cell & Developmental Biology·Xiping ChengHaoxing Xu
Sep 11, 2003·Head & Neck·Boban M ErovicCsilla Neuchrist
Dec 21, 2018·Cellular and Molecular Life Sciences : CMLS·Stephanie PaoneIvan K H Poon

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