PMID: 18435281Apr 26, 2008Paper

Expression of quail vascular endothelial growth factor receptor quek-1 extracellular domain 2-4 loop cDNA in Pichia pastoris

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Peng DiaoYuquan Wei

Abstract

The extracellular domain 2-4 loop cDNA of quail vascular endothelial growth factor receptor quek1 (qVEGFR2) was obtained from plasmid carrying qVEGFR2 by PCR. Then it was cloned into expression vector pPICZalphaA of Pichia pastoris. To construct recombinant expression plasmid pPICZalphaA-qVEGFR2, linearized pPICZalphaA-qVEGFR2 with SacI was transformed to electroporated Pichia pastoris GS115. Subsequently, positive clone was selected by PCR and its phenotype was determined. SDSPAGE and Western blot assays of culture medium from a methanol-induced expression strain demonstrated that recombinant qVEGFR2 proteins were expressed and secreted into the culture medium. These results could provide a basis for further researches on tumor protein vaccine as well as for the preparation of tumor protein vaccine with Pichia pastoris.

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

Related Papers

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Jianling WangLianxiang Du
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Zhi-Qing HuangYong-Fu Chen
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Xiaobo DuYuquan Wei
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Yucheng SunHongwei Gao
Wei sheng wu xue bao = Acta microbiologica Sinica
Q WangX Wang
© 2022 Meta ULC. All rights reserved