Expression of Rab3D N135I inhibits regulated secretion of ACTH in AtT-20 cells

The Journal of Cell Biology
G BaldiniA M Martelli

Abstract

Rab proteins are small molecular weight GTPases that control vesicular traffic in eucaryotic cells. A subset of Rab proteins, the Rab3 proteins are thought to play an important role in regulated exocytosis of vesicles. In transfected AtT-20 cells expressing wild-type Rab3D, we find that a fraction of the protein is associated with dense core granules. In the same cells, expression of a mutated isoform of Rab3D, Rab3D N135I, inhibits positioning of dense core granules near the plasma membrane, blocks regulated secretion of mature ACTH, and impairs association of Rab3A to membranes. Expression of Rab3D N135I does not change the levels of ACTH precursor or the efficiency with which the precursor is processed into ACTH hormone and packaged into dense core granules. We also find that cells expressing mutated Rab3D differentiate to the same extent as untransfected AtT-20 cells. We conclude that expression of Rab3D N135I specifically impairs late membrane trafficking events necessary for ACTH hormone secretion.

References

Nov 19, 1992·Nature·A F OberhauserJ M Fernandez
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P J PadfieldJ D Jamieson
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G BaldiniH F Lodish
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K KatoC J Der
Nov 1, 1991·The Journal of Cell Biology·M MatteoliP De Camilli
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G Fischer von MollardT C Südhof
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F DarchenD Scherman
Feb 1, 1988·The Journal of Cell Biology·L MatsuuchiR B Kelly
Apr 1, 1987·Bioscience Reports·T SchäferM M Burger
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N TouchotA Tavitian
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B Gumbiner, R B Kelly
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·G BaldiniH F Lodish
Jun 9, 1994·Nature·M GeppertT C Südhof
Dec 1, 1994·The Journal of Cell Biology·P E SchererH F Lodish
Jan 1, 1994·Annual Review of Biochemistry·M K Bennett, R H Scheller
Aug 1, 1994·Current Opinion in Cell Biology·S R Pfeffer
Apr 1, 1994·Trends in Biochemical Sciences·G Fischer von MollardR Jahn
May 1, 1994·The Journal of Cell Biology·E WeberD R Abrahamson
Nov 1, 1993·Neuron·K Simons, M Zerial
Jul 1, 1993·Molecular Biology of the Cell·J K NgseeR H Scheller
Mar 22, 1996·The Journal of Biological Chemistry·E WeberK L Kirk
Jun 1, 1996·The Journal of Cell Biology·R A ChavezH P Moore

❮ Previous
Next ❯

Citations

Nov 17, 2010·Cellular and Molecular Neurobiology·Tanja Kögel, Hans-Hermann Gerdes
Mar 21, 2002·The Biochemical Journal·Nicholas J CartelMartin Post
Jan 27, 2004·Molecular Biology of the Cell·Evelina ChieregattiGiulia Baldini
Aug 23, 2002·Molecular and Cellular Biology·Dietmar RiedelReinhard Jahn
Jun 17, 2004·Molecular and Cellular Biology·Daniela SpanoRoberto Di Lauro
Jun 1, 2005·Molecular and Cellular Biology·Nathan J PavlosMing H Zheng
Jun 12, 2004·American Journal of Respiratory Cell and Molecular Biology·Christopher M EvansBurton F Dickey
Aug 2, 2008·Molecular Endocrinology·Joshua J Park, Y Peng Loh
Mar 26, 2013·PloS One·Tanja KögelHans-Hermann Gerdes
Sep 21, 2001·Biochemical and Biophysical Research Communications·A PiiperS Zeuzem
Aug 22, 2001·FEBS Letters·J C GevreyJ Abello
Oct 21, 2003·Journal of Cellular Physiology·Danieele NguyenDanielle Ngyen
Oct 3, 2003·The Journal of Biological Chemistry·Xuequn ChenJohn A Williams
Dec 15, 2006·The Journal of Biological Chemistry·Sameer MohammadGiulia Baldini
Mar 5, 2002·The Journal of Biological Chemistry·Xuequn ChenJohn A Williams
Mar 29, 2003·Physiological Reviews·Robert D Burgoyne, Alan Morgan
Mar 30, 2001·The Journal of Biological Chemistry·S ChenA E Ting
Dec 22, 2007·Journal of Cell Science·Daniel J MetcalfDaniel F Cutler
Dec 22, 1999·Biochemical and Biophysical Research Communications·K ValentijnJ D Jamieson

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPases
Transfection
electrophoresis
confocal microscopy
GTPase

Software Mentioned

Videoplan Image
Mop
Adobe Photoshop

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.