Expression of RANTES mRNA and protein in airways of patients with mild asthma

American Journal of Respiratory and Critical Care Medicine
N BerkmanK F Chung

Abstract

Chemoattractant cytokines (chemokines) such as RANTES, are potent chemoattractants for eosinophils, T lymphocytes, and monocyte/macrophages. We examined RANTES mRNA and protein expression in bronchial biopsies and bronchoalveolar lavage (BAL) cells from patients with mild asthma on beta 2-adrenergic agonist therapy only. Using quantitative polymerase chain reaction (PCR), mean RANTES starting cDNA was 110 +/- 18 fg/pg beta-actin in asthmatics compared with 33.7 +/- 11.0 fg/pg in normals (p < 0.003) but there was no significant difference in RANTES mRNA expression in BAL cells between the two groups. Immunohistochemical staining of cryostat sections of bronchial biopsies with an anti-RANTES antibody using peroxidase antiperoxidase method revealed constitutive staining in airway epithelial cells, airway smooth muscle, and subepithelial cells. However, there was no difference in the number of RANTES-staining cells between normal subjects and asthmatics despite significantly increased numbers of CD4+ T-cells, CD68+ macrophages, and MBP+ eosinophils in mucosal biopsies obtained from asthmatics. Double-staining revealed expression of RANTES in a proportion of CD3+ T lymphocytes. Thus, RANTES is constitutively expressed in the airways...Continue Reading

Citations

Mar 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L M Gale, S R McColl
Mar 20, 2002·Current Allergy and Asthma Reports·L Cameron, Q Hamid
Jul 1, 2008·Current Allergy and Asthma Reports·Omar Tliba, Reynold A Panettieri
Apr 28, 2004·Respiratory Physiology & Neurobiology·Limei ZhouMarc B Hershenson
Jul 13, 2002·Pharmacology & Therapeutics·Y Riffo-Vasquez, D Spina
Oct 18, 2003·Cytokine & Growth Factor Reviews·Peter J Barnes
Nov 20, 2001·Current Opinion in Pharmacology·A L Lazaar, R A Panettieri
Oct 1, 2003·Respiratory Physiology & Neurobiology·Reynold A Panettieri
Oct 1, 2003·Respiratory Physiology & Neurobiology·Stuart J Hirst
Mar 22, 2002·Nature Reviews. Immunology·N W Lukacs
Aug 8, 2001·Immunology and Cell Biology·I M Adcock, G Caramori
Feb 15, 2001·The New England Journal of Medicine·W W Busse, R F Lemanske
May 18, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Younes J ErrahaliHenry J Lee
Aug 24, 1999·Thorax·K F Chung, P J Barnes
Oct 15, 2008·Annual Review of Physiology·Omar Tliba, Reynold A Panettieri
Nov 17, 1998·American Journal of Respiratory and Critical Care Medicine·L A HartK F Chung
Nov 9, 2000·American Journal of Respiratory and Critical Care Medicine·S LimK F Chung
Nov 9, 2000·American Journal of Respiratory and Critical Care Medicine·M SugaM Liu
May 2, 1998·American Journal of Respiratory Cell and Molecular Biology·N KodamaY Kawakami
Mar 11, 2006·American Journal of Respiratory Cell and Molecular Biology·Toshiyuki KoyaErwin W Gelfand
Sep 28, 2010·American Journal of Respiratory Cell and Molecular Biology·Ming ZhuStephanie A Shore
Nov 5, 2008·The Journal of Clinical Investigation·Peter J Barnes
Apr 23, 2013·Mediators of Inflammation·Petra Seidel, Michael Roth
Apr 16, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·N KrugR Pabst
Jul 19, 2005·Expert Opinion on Investigational Drugs·James E Pease, Richard Horuk
Mar 27, 2012·The Journal of Allergy and Clinical Immunology·Leonard B BacharierMario Castro
May 1, 2010·Archives of Medical Research·Yong-Gang ZhangHong Fan
Jan 6, 2009·Pulmonary Pharmacology & Therapeutics·Gautam DameraReynold A Panettieri
Dec 31, 2008·Pulmonary Pharmacology & Therapeutics·Jeremy A HirotaThai Tran
Dec 16, 2006·European Journal of Pharmacology·Dae Yong KimJai Youl Ro
May 29, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L ChachiY Amrani
Jan 12, 2000·The Journal of Allergy and Clinical Immunology·Q A Hamid, E M Minshall
Oct 10, 2006·Biochemical and Biophysical Research Communications·Koutarou MiyabayashiMasashi Kobayashi
Mar 9, 1999·The Journal of Allergy and Clinical Immunology·R A TahaQ A Hamid
Jun 9, 1999·The Journal of Allergy and Clinical Immunology·M HoshinoA Koya
Jun 20, 2015·Pflügers Archiv : European journal of physiology·Amy M SpinelliHarold A Singer
Jul 13, 2005·Pharmacology & Therapeutics·Cory M HogaboamKaren F Buckland
Jul 25, 2000·Clinics in Chest Medicine·S MuroQ A Hamid
Jan 16, 2013·Journal of Parasitology Research·Robson Da Paixão De SouzaMaria Ilma Araujo
Oct 5, 2013·Expert Opinion on Therapeutic Targets·Rafael Elias MarquesMauro Martins Teixeira
Oct 12, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·D Diaz-SanchezA Saxon
Nov 28, 2007·Annual Review of Pathology·Marc B HershensonJulian Solway

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.

Related Papers

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
L Pang, A J Knox
American Journal of Respiratory and Critical Care Medicine
O GhaffarB Lamkhioued
American Journal of Respiratory and Critical Care Medicine
J V FahyJ S Abrams
© 2021 Meta ULC. All rights reserved