Expression of RAP1B is associated with poor prognosis and promotes an aggressive phenotype in gastric cancer

Oncology Reports
Ya YangXiao-Yi Duan

Abstract

Metastasis is the major cause of death among gastric cancer (GC) patients, and altered expression of Ras-related protein RAP1B is associated with cancer development. The present study assessed RAP1B expression ex vivo and the effect of hypoxia‑induced RAP1B overexpression on the promotion of the metastatic potential of GC cells in vitro. Immunohistochemistry was used to detect the expression of RAP1B and hypoxia‑inducible factor-1α (HIF-1α) in 178 GC tissue specimens. GC cell lines were used to assess the effects of hypoxia and RAP1B knockdown with RAP1B small interfering RNA (siRNA). Tumor cell viability was detected by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, invasion capacity was evaluated by a Transwell assay, and gene expression was determined by qRT-PCR and western blotting. The data showed that expression levels of RAP1B and HIF-1α proteins were high in the GC tissue specimens, and RAP1B expression was significantly associated with tumor-node-metastasis (TNM) stage and tumor size, while HIF-1α expression was significantly associated with TNM stage, Borrmann type and tumor differentiation. Moreover, RAP1B expression was associated with HIF-1α expression (r=0.547, P<0.001). The expressi...Continue Reading

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Citations

May 17, 2018·British Journal of Haematology·Susanne Bram EdnerssonPer-Ola Andersson
Mar 27, 2020·Frontiers in Oncology·Qiang WangXiangqian Guo
Aug 11, 2020·Journal of Experimental & Clinical Cancer Research : CR·Qing HanZhiwang Song
Sep 11, 2020·Biomedicines·Chin-King LooiChun-Wai Mai

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