Expression of RASSF6 in kidney and the implication of RASSF6 and the Hippo pathway in the sorbitol-induced apoptosis in renal proximal tubular epithelial cells

Journal of Biochemistry
Kanchanamala WithanageYutaka Hata

Abstract

RASSF6, a member of RASSF tumour suppressor proteins, binds to mammalian Ste20-like kinases (MST1/2), core kinases of the proapoptotic Hippo pathway and cooperates with the Hippo pathway to induce apoptosis. We originally identified RASSF6 as a putative interactor of membrane-associated guanylate kinase inverted (MAGI)-1 by the yeast two-hybrid screening. We used human kidney cDNA library for the screening. MAGI-1 is abundantly expressed in kidney and is a core component of the slit diaphragm. These findings suggest that RASSF6 is expressed in kidney. However, the function of RASSF6 in kidney is not yet studied. We performed this study to confirm the interaction of RASSF6 with MAGI-1, to analyse the expression of RASSF6 in kidney and to gain insight into the function of RASSF6 in kidney. RASSF6 binds to PDZ domains of MAGI-1 through its C-terminal PDZ-binding motif and is coimmunoprecipitated with MAGI-1 from rat liver. RASSF6 is localized in normal kidney glomerulus but disappears when the slit diaphragm is disrupted in nephrotic kidney. RASSF6 is also localized on apical membranes in renal proximal tubular epithelial cells. We demonstrated that RASSF6 as well as the Hippo pathway are involved in the sorbitol-induced apoptosis...Continue Reading

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Citations

Dec 23, 2015·Cancers·Hiroaki IwasaYutaka Hata
Sep 2, 2014·Frontiers in Endocrinology·François BouteauLaure Bonnaud-Ponticelli
Jan 22, 2018·Cellular and Molecular Life Sciences : CMLS·Hiroaki IwasaYutaka Hata
Dec 2, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Xiaoyin XuYutaka Hata
Jun 13, 2018·Molecular and Cellular Biology·Shakhawoat HossainYutaka Hata
Sep 5, 2013·The Journal of Biological Chemistry·Hiroaki IwasaYutaka Hata
Sep 24, 2021·Journal of Cellular Physiology·Aimei LiuIrma Ares

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