Expression of rat thyrotropin-releasing hormone (TRH) gene in TRH-producing tissues of transgenic mice requires sequences located in exon 1

Endocrinology
W BalkanB A Roos

Abstract

TRH, an amidated tripeptide secreted by certain hypothalamic neurons, is a principal regulator of TSH secretion and thyroid hormone release. TRH is also produced by other neurons in the central nervous system, where it appears to function as a neuromodulator or neurotransmitter, and by certain endocrine cells, where it may act as an autocrine or paracrine factor. The genomic organization of the rat TRH (rTRH) gene is well understood; however, the domains of the rTRH gene that regulate expression are less well characterized. We observed that the region between -47 and +6 of the rTRH gene (relative to the transcription start site at +1) was active in CA-77 cells, a medullary thyroid carcinoma cell line model of TRH production, but was not active in transgenic mice. Inclusion of most of exon 1 (84 out of 103 bp; -47 to +84) increased promoter activity in CA-77 cells and was active in transgenic mice, principally in tissues that normally express the TRH gene. Further lengthening of the 5' end to -243, -547, or -776 retained this expression in TRH-producing tissues in transgenic mice, while further increasing activity in CA-77 cells. These results suggest that cis element(s) located within exon 1 are necessary for the expression of ...Continue Reading

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Citations

May 17, 2011·BMC Genomics·Magdalena Guerra-CrespoJean-Louis Charli
Dec 25, 2010·Molecular and Cellular Endocrinology·Carlos Pérez-MonterLeonor Pérez-Martínez
Jan 27, 2011·Biochimica Et Biophysica Acta·Antonieta Cote-VélezPatricia Joseph-Bravo
Apr 20, 2010·Molecular and Cellular Endocrinology·Stéphanie DecherfBarbara A Demeneix
Dec 3, 2014·Molecular and Cellular Endocrinology·Miriam Martínez-ArmentaLeonor Pérez-Martínez
Nov 1, 2005·Developmental Cell·Maria V GustafssonMaria Bondesson
Oct 26, 1999·Endocrine Reviews·E A Nillni, K A Sevarino
Jun 29, 2002·Molecular Endocrinology·Hajer GuissoumaBarbara A Demeneix
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Dec 5, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H GuissoumaB A Demeneix
Sep 9, 2017·Nature Communications·Helge Müller-FielitzMarkus Schwaninger
Jan 7, 2020·Endocrine·Ludmilla Ferreira CardosoLéa Maria Zanini Maciel

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