Expression of recombinant human ceruloplasmin--an absolute requirement for splicing signals in the expression cassette

FEBS Letters
M RafiqR W Evans

Abstract

We report the successful expression of recombinant human ceruloplasmin which was made possible by inclusion of splicing signals in the expression vector. Ceruloplasmin cDNA expressed from the vector pNUT in baby hamster kidney cells gave protein yields of 0.03 mg/l which increased to 15 mg/l with splicing signals present. The defect in expression from the intronless cDNA is due to complete retention of ceruloplasmin mRNA in cell nuclei. The block to cytoplasmic export is alleviated by splicing signals, allowing full expression of the mRNA.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P GrussG Khoury
Dec 1, 1979·Cell·D H Hamer, P Leder
Nov 1, 1977·Arthritis and Rheumatism·J D GitlinD Gitlin
Dec 15, 1991·The Biochemical Journal·J L JaegerJ D Gitlin
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M L KoschinskyR T MacGillivray
Oct 1, 1988·Molecular and Cellular Biology·A R Buchman, P Berg
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R L BrinsterR D Palmiter
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W R ChurchD N Fass
Jan 20, 1995·The Journal of Biological Chemistry·D M De SilvaJ Kaplan

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Citations

Mar 10, 2011·Molecular Biotechnology·Natasa SkokoEmanuele Buratti
Apr 26, 2003·Trends in Biochemical Sciences·Hervé Le HirMelissa J Moore
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patricia ValenciaRobin Reed
Apr 14, 2004·Journal of Virology·Rozanne M Sandri-Goldin
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·M J Luo, R Reed
Mar 2, 2016·Biologicals : Journal of the International Association of Biological Standardization·Mohammad Reza SamAram Mokarizadeh
Feb 26, 2016·Journal of Cell Science·Chen Wang, Ben G Szaro

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