Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

Chemical Communications : Chem Comm
Steven P HanlonMargit Winkler

Abstract

A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.

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Citations

Jan 16, 2016·ACS Chemical Biology·Filippo FiorentiniAndrea Mattevi
Mar 30, 2016·Journal of Biotechnology·Márcia Ferreira AntunesMargit Winkler
Jun 13, 2015·Microbial Cell Factories·Martina GeierMargit Winkler
Sep 30, 2016·Expert Opinion on Drug Metabolism & Toxicology·Ian R Phillips, Elizabeth A Shephard
Mar 31, 2018·Angewandte Chemie·Margit WinklerAnton Glieder
Jul 19, 2019·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Ian R Phillips, Elizabeth A Shephard
Nov 11, 2017·Organic & Biomolecular Chemistry·Elena LenciAndrea Trabocchi
Aug 14, 2019·Microbial Cell Factories·Chongliang Gao, Tingjie Zheng
Mar 22, 2020·Microbial Cell Factories·Gianluca CatucciSheila J Sadeghi
Jun 7, 2020·Applied Microbiology and Biotechnology·Carolin MüggeDirk Tischler
Jul 9, 2014·Journal of Medicinal Chemistry·Gabriele CrucianiMassimo Baroni
Aug 8, 2017·ACS Chemical Biology·Filippo FiorentiniAndrea Mattevi

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