PMID: 19912821Oct 1, 1991Paper

Expression of recombinant rat ciliary neurotrophic factor in Escherichia coli and its synergy with basic fibroblast growth factor

Molecular and Cellular Neurosciences
M E Gurney, H Yamamoto

Abstract

Rat CNTF was expressed in Escherichia coli using a T7 RNA polymerase vector system. rCNTF was obtained as an insoluble aggregate at levels approaching 70% of total E. coli protein. After extraction, renaturation, and purification by anion-exchange chromatography and gel filtration the yield of biologically active rCNTF was nearly 0.5 mg/ml of E. coli culture broth. The effects of rCNTF and bFGF on cultured chick ciliary neurons were compared. bFGF was primarily neuritogenic and did not support ciliary neuron survival beyond 2 days in culture. rCNTF induced neuritogenesis more slowly and supported ciliary neuron survival in culture for a longer period. bFGF strongly potentiated the effect of rCNTF. Without bFGF, the ED(50) for rCNTF was 102 +/- 25 pg/ml. With bFGF, the potency of rCNTF increased to an ED(50) of 42 +/- 6 pg/ml. Chick nodose neurons also responded to rCNTF and their response was potentiated by bFGF. Thus, bFGF modulates the response of ciliary and nodose neurons to CNTF in vitro and may have a similar effect in vivo.

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Citations

Jul 5, 2011·Physiology & Behavior·Éadaoin W GriffinAine M Kelly
Aug 13, 2010·Psychoneuroendocrinology·B A A BusR C Oude Voshaar
Sep 7, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Ritsuko Katoh-SembaAtsuo Nakayama
Dec 5, 2012·Acta Histochemica Et Cytochemica·Keiichi Tsukinoki, Juri Saruta
Feb 1, 1992·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·M M McGrane, R W Hanson

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