Expression of recombinant staphylokinase, a fibrin-specific plasminogen activator of bacterial origin, in potato (Solanum tuberosum L.) plants.

World Journal of Microbiology & Biotechnology
Aneta GerszbergAndrzej K Kononowicz

Abstract

One of the most dynamically developing sectors of green biotechnology is molecular farming using transgenic plants as natural bioreactors for the large scale production of recombinant proteins with biopharmaceutical and therapeutic values. Such properties are characteristic of certain proteins of bacterial origin, including staphylokinase. For many years, work has been carried out on the use of this protein in thrombolytic therapy. In this study, transgenic Solanum tuberosum plants expressing a CaMV::sak-mgpf-gusA gene fusion, were obtained. AGL1 A. tumefaciens strain was used in the process of transformation. The presence of the staphylokinase gene was confirmed by PCR in 22.5% of the investigated plants. The expression of the fusion transgene was detected using the β-glucuronidase activity assay in 32 putative transgenic plants. Furthermore, on the basis of the GUS histochemical reaction, the transgene expression pattern had a strong, constitutive character in seven of the transformants. The polyacrylamide gel electrophoresis of a protein extract from the SAK/PCR-positive plants, revealed the presence of a119 kDa protein that corresponds to that of the fusion protein SAK-mGFP-GUSA. Western blot analysis, using an antibody aga...Continue Reading

References

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Citations

Feb 26, 2013·International Journal of Molecular Sciences·Maria Manuela RiganoAmalia Barone
Jun 6, 2016·World Journal of Microbiology & Biotechnology·Katarzyna Hnatuszko-KonkaAndrzej K Kononowicz
Nov 16, 2019·International Journal of Biological Macromolecules·Min WangDonggang Xu

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Methods Mentioned

BETA
transgenic
PCR
electrophoresis
transgenics

Software Mentioned

Image Master VDS

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