Expression of Reg family genes in the gastrointestinal tract of mice treated with indomethacin

American Journal of Physiology. Gastrointestinal and Liver Physiology
Chao SunHiroto Miwa

Abstract

Regenerating gene (Reg) family proteins, which are classified into four types, commonly act as trophic and/or antiapoptotic factors in gastrointestinal (GI) diseases. However, it remains unclear how these proteins coordinate their similar roles under such pathophysiological conditions. Here, we investigated the interrelationships of Reg family gene expression with mucosal cell proliferation and apoptosis in nonsteroidal anti-inflammatory drug (NSAID)-induced GI injury. GI injury was induced by subcutaneous injection of indomethacin into Reg I knockout (KO) and wild-type (WT) mice, and its severity was scored histopathologically. Temporal changes in the expression of Reg family genes, mucosal proliferation, and apoptosis were evaluated throughout the GI tract by real-time RT-PCR, Ki-67 immunoreactivity, and TUNEL assay, respectively. Reg I, Reg III family, and Reg IV were predominantly expressed in the upper, middle, and lower GI mucosa, respectively. Expression of Reg I and Reg III family genes was upregulated in specific portions of the GI tract after indomethacin treatment. Ki-67-positive epithelial cells were significantly decreased in the gastric and small-intestinal mucosa of Reg I KO mice under normal conditions. After tr...Continue Reading

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Citations

Apr 8, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Yoshitaka KitayamaHiroto Miwa
Mar 19, 2016·Development·Valeria Fernandez ValloneMarie-Isabelle Garcia
Mar 25, 2017·Frontiers in Microbiology·Christelle F IskandarAnne-Marie Revol-Junelles
Aug 29, 2020·International Journal of Molecular Sciences·Ying RanHiroto Miwa
Jun 20, 2020·World Journal of Gastroenterology : WJG·Jodi-Ann EdwardsChongmin Huan
Nov 24, 2021·Molecular Biology Reports·Liying WangFengyuan Che

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