Abstract
Regenerating gene (Reg) family proteins, which are classified into four types, commonly act as trophic and/or antiapoptotic factors in gastrointestinal (GI) diseases. However, it remains unclear how these proteins coordinate their similar roles under such pathophysiological conditions. Here, we investigated the interrelationships of Reg family gene expression with mucosal cell proliferation and apoptosis in nonsteroidal anti-inflammatory drug (NSAID)-induced GI injury. GI injury was induced by subcutaneous injection of indomethacin into Reg I knockout (KO) and wild-type (WT) mice, and its severity was scored histopathologically. Temporal changes in the expression of Reg family genes, mucosal proliferation, and apoptosis were evaluated throughout the GI tract by real-time RT-PCR, Ki-67 immunoreactivity, and TUNEL assay, respectively. Reg I, Reg III family, and Reg IV were predominantly expressed in the upper, middle, and lower GI mucosa, respectively. Expression of Reg I and Reg III family genes was upregulated in specific portions of the GI tract after indomethacin treatment. Ki-67-positive epithelial cells were significantly decreased in the gastric and small-intestinal mucosa of Reg I KO mice under normal conditions. After tr...Continue Reading
References
Apr 18, 1998·Gastroenterology·E M OrtizJ L Iovanna
Nov 14, 2000·Physiological Genomics·B K DieckgraefeC A Harrington
Feb 22, 2001·Human Molecular Genetics·I C LawranceS Chakravarti
Oct 12, 2001·Journal of Physiology, Paris·A TarnawskiB Soreghan
Dec 12, 2002·Diabetes·Michiaki UnnoHiroshi Okamoto
Jun 7, 2003·Inflammatory Bowel Diseases·Hitoshi OgawaIwao Sasaki
Dec 24, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hirokazu FukuiRobert M Genta
Jul 6, 2004·Journal of Gastroenterology·Yoshikazu KinoshitaTsutomu Chiba
Mar 15, 2005·Gastroenterology·Akira SekikawaTsutomu Chiba
May 26, 2005·Gut·A SekikawaT Chiba
Aug 24, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hanh-Tu LieuLaurence Christa
Aug 22, 2006·Alimentary Pharmacology & Therapeutics·L LaineR W Dubois
Feb 3, 2007·Cancer Science·Shigeo Takaishi, Timothy C Wang
Nov 1, 2008·The Journal of Pathology·A AbeT Fujimori
Jan 23, 2009·Digestive Diseases and Sciences·Marleen T J van AmptingIngeborg M J Bovee-Oudenhoven
Nov 11, 2009·Gastroenterology·Kumar S BishnupuriBrian K Dieckgraefe
Jan 13, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Akira SekikawaTakahiro Fujimori
May 29, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hiroshi ImaokaYoshikazu Kinoshita
Oct 19, 2010·Gastroenterology Clinics of North America·Carmelo Scarpignato, Richard H Hunt
Nov 3, 2010·Journal of Pharmacological Sciences·Koji TakeuchiKikuko Amagase
Oct 15, 2011·Science·Shipra VaishnavaLora V Hooper
Jun 26, 2012·Immunity·Yuping LaiRichard L Gallo
Dec 18, 2013·Journal of Proteome Research·Mindy PorterfieldLance Wells
Citations
Apr 8, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Yoshitaka KitayamaHiroto Miwa
Mar 19, 2016·Development·Valeria Fernandez ValloneMarie-Isabelle Garcia
Mar 25, 2017·Frontiers in Microbiology·Christelle F IskandarAnne-Marie Revol-Junelles
Mar 15, 2019·Scientific Reports·Xin XuHiroto Miwa
Nov 30, 2019·Mediators of Inflammation·Xin XuHiroto Miwa
Aug 29, 2020·International Journal of Molecular Sciences·Ying RanHiroto Miwa
Nov 23, 2017·Biological Research·Haijie XingYaofeng Han
Feb 6, 2019·Molecular Medicine Reports·Yoshihito InoueHiroto Miwa
Jun 20, 2020·World Journal of Gastroenterology : WJG·Jodi-Ann EdwardsChongmin Huan
Jan 30, 2021·International Immunopharmacology·Hongli MaoFucheng He
May 1, 2021·Pathogens·Heihachiro NishimuraHiroto Miwa
Oct 12, 2021·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hiroshi Okamoto, Shin Takasawa
Nov 24, 2021·Molecular Biology Reports·Liying WangFengyuan Che