Expression of RHOGTPase regulators in human myometrium

Reproductive Biology and Endocrinology : RB&E
Margaret O'BrienTerry J Smith

Abstract

RHOGTPases play a significant role in modulating myometrial contractility in uterine smooth muscle. They are regulated by at least three families of proteins, RHO guanine nucleotide exchange factors (RHOGEFs), RHOGTPase-activating proteins (RHOGAPs) and RHO guanine nucleotide inhibitors (RHOGDIs). RHOGEFs activate RHOGTPases from the inactive GDP-bound to the active GTP-bound form. RHOGAPs deactivate RHOGTPases by accelerating the intrinsic GTPase activity of the RHOGTPases, converting them from the active to the inactive form. RHOGDIs bind to GDP-bound RHOGTPases and sequester them in the cytosol, thereby inhibiting their activity. Ezrin-Radixin-Moesin (ERM) proteins regulate the cortical actin cytoskeleton, and an ERM protein, moesin (MSN), is activated by and can also activate RHOGTPases. We therefore investigated the expression of various RHOGEFs, RHOGAPs, a RHOGDI and MSN in human myometrium, by semi-quantitative reverse transcription PCR, real-time fluorescence RT-PCR, western blotting and immunofluorescence microscopy. Expression of these molecules was also examined in myometrial smooth muscle cells. ARHGEF1, ARHGEF11, ARHGEF12, ARHGAP5, ARHGAP24, ARHGDIA and MSN mRNA and protein expression was confirmed in human myometr...Continue Reading

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Citations

Aug 21, 2013·Reproductive Biology and Endocrinology : RB&E·Margaret O'BrienTerry J Smith
Jun 1, 2010·Reproductive Biology and Endocrinology : RB&E·Margaret O'BrienTerry J Smith
Nov 12, 2015·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·A Awaad, A A Seleem
Sep 20, 2012·Reproductive Sciences·Phukphon MunglueSajeera Kupittayanant
May 12, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Fabiane de SantiEstela Sasso-Cerri
Jun 10, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Fengyin LiShixian Zhou
Jul 11, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·J Lartey, A López Bernal
Jan 30, 2021·Cell and Tissue Research·MacKinsey Johnson, Chishimba Nathan Mowa
Oct 13, 2009·Cellular Signalling·Annica K B Gad, Pontus Aspenström

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Methods Mentioned

BETA
GTPases
nucleotide exchange
GTPase
biopsies
hysterectomy
hysterectomies
PCR
electrophoresis
protein assay
fluorescence

Software Mentioned

GraphPad Prism
AlphaEaseFC
SPSS
SPSS Package for the Social Sciences

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