Expression of RNA from developmentally important genes in preimplantation bovine embryos produced in TCM supplemented with BSA.

Journal of Reproduction and Fertility
C WrenzyckiH Niemann

Abstract

This study investigated the effects of a semi-defined culture system on the temporal pattern of expression of RNA from genes involved in compaction and cavitation: gap junction protein connexin43 (Cx43), desmosomal glycoproteins desmoglein 1 (Dg 1), desmocollins I, II and III (Dc I, Dc II, Dc III), desmosomal protein plakophilin (Plako); metabolism glucosetransporter-1 (Glut-1); RNA processing poly(A)polymerase (PolyA); heat shock protein 70.1 (HSP); and trophoblastic function trophoblast protein (TP) in bovine oocytes and embryos generated in vitro using TCM199 supplemented with BSA as the culture medium. Morulae and blastocysts derived in vivo were collected from superovulated heifers and also used for this study. Poly(A)+ RNA was extracted from pools of 20-50 oocytes or embryos, analysed by reverse transcription-polymerase chain reaction and the amplified fragments were verified by sequencing. Assays were repeated at least three times for each developmental stage and provided consistent results in all replicates. In bovine embryos produced in vitro, mRNA encoding Cx43 was detectable up to the morula stage, whereas blastocysts and hatched blastocysts did not express this gene. No transcripts were found for Dg 1 and Dc I throu...Continue Reading

Citations

Jan 6, 2001·Molecular Reproduction and Development·A S LequarréA Van Langendonckt
May 10, 2005·Theriogenology·Alexandre Tavares Duarte de OliveiraJosé Luiz Rodrigues
Feb 24, 2001·Theriogenology·D H Betts, W A King
Mar 29, 2000·Theriogenology·C E Rexroad, A M Powell
Jan 30, 2004·Reproductive Biomedicine Online·Christine Wrenzycki, Heiner Niemann
Dec 6, 2005·BMC Developmental Biology·Karen GoossensLuc J Peelman
Aug 26, 2011·Reproductive Biology and Endocrinology : RB&E·Gautam Kumar DebIl Keun Kong
Mar 12, 2009·The Journal of Reproduction and Development·Ken Sawai
May 18, 2000·Biochemical and Biophysical Research Communications·A Navarrete SantosB Fischer
Jul 13, 2012·Zygote : the Biology of Gametes and Early Embryos·Eva AbeleChristine Wrenzycki
Feb 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Nadia Gopichandran, Henry J Leese
Dec 23, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Michael HoelkerDawit Tesfaye
Apr 14, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Isabelle CôtéClaude Robert
Oct 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Anne Navarrete SantosBernd Fischer
Jul 21, 2009·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Sarah TonackAnne Navarrete Santos
Jul 6, 2007·Theriogenology·K H S CampbellJ Xhu
Mar 17, 2009·Molecular Reproduction and Development·M N PurperaK R Bondioli
Feb 18, 2011·Reproduction in Domestic Animals = Zuchthygiene·E DovolouD Rizos
Mar 15, 2008·Biochimica Et Biophysica Acta·Judith J Eckert, Tom P Fleming
May 21, 2013·Theriogenology·N N CostaO M Ohashi
Jul 11, 2006·Zygote : the Biology of Gametes and Early Embryos·R M Garcia-GarciaM J Cocero
Sep 24, 1999·Biology of Reproduction·R BoniB Dale
May 28, 2004·Biology of Reproduction·A J HarveyJ G Thompson
Oct 23, 2002·Biology of Reproduction·Claude RobertMarc-André Sirard

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