Expression of rotavirus VP6 protein: a comparison amongst Escherichia coli, Pichia pastoris and Hansenula polymorpha

FEMS Yeast Research
H BredellW H van Zyl

Abstract

During this study. we successfully expressed a codon-optimized gene for rotavirus VP6 protein intracellularly in two methylotrophic yeasts, Pichia pastoris and Hansenula polymorpha, during methanol induction. Expressions were performed in shake flasks and subsequently scaled-up to 1.3 L bioreactors. The yields obtained in the yeasts were compared with that observed in Escherichia coli. Despite producing the lowest biomass levels of all the expression systems in shake flasks, the highest VP6 concentration was obtained with E. coli. In shake flasks, P. pastoris yielded higher volumetric levels of VP6 than H. polymorpha, but specific production of VP6 was approximately similar in both yeasts. In the controlled environment of bioreactors, yeast strains attained typical high cell densities, but also increased VP6 production compared to all shake flask cultures. Unlike in shake flask expressions, H. polymorpha outperformed both P. pastoris as well as E. coli during bioreactor cultivation. VP6 production was in all three expression systems growth-associated. In contrast to yeast expressions, bacterial expressed VP6 protein was found to be insoluble upon analysis. This is the first report of VP6 expressed in methylotrophic yeast and ho...Continue Reading

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Citations

May 24, 2019·Frontiers in Bioengineering and Biotechnology·João Heitor Colombelli Manfrão-NettoNádia Skorupa Parachin
Jul 2, 2021·Microbial Cell Factories·Aravind MadhavanParameswaran Binod
Oct 12, 2021·PloS One·Dzhirgala V ShamadykovaGalina V Pavlova
Nov 9, 2021·Applied Microbiology and Biotechnology·Xiaoxin ZhaiYongjin J Zhou

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